CRE-decoy oligonucleotide-inhibition of gene expression and tumor growth
CRE-decoy oligonucleotide-inhibition of gene expression and tumor growth
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DOI：
10.1023/a:1007144618589
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发表时间：
2000-09-01
影响因子：
4.3
通讯作者：
Cho, YS
中科院分区：
文献类型：
--
作者：
Cho-Chung, YS;Park, YG;Cho, YS
对靶转录因子具有高亲和力的核酸分子可以作为诱饵顺式元件引入细胞中以结合这些因子并改变基因表达。这篇综述讨论了一种合成的单链回文寡核苷酸，其自身杂交形成双链体/发夹，并与cAMP反应元件（CRE）增强子竞争结合转录因子。这种寡核苷酸抑制CRE和Ap-1指导的基因转录，并在体外和体内广泛的癌细胞中促进生长抑制，而不会对正常细胞生长产生不利影响。这里提出的证据表明，CRE-decoy寡核苷酸可以通过调节cAMP应答基因的表达来提供对抗癌症、病毒性疾病和其他病理状况的强有力的新手段。
Nucleic acid molecules with high affinities for a target transcription factor can be introduced into cells as decoy cis-elements to bind these factors and alter gene expression. This review discusses a synthetic single-stranded palindromic oligonucleotide, which self-hybridizes to form a duplex/hairpin and competes with cAMP response element (CRE) enhancers for binding transcription factors. This oligonucleotide inhibits CRE- and Ap-1-directed gene transcription and promotes growth inhibition in vitro and in vivo in a broad spectrum of cancer cells, without adversely affecting normal cell growth. Evidence presented here suggests that the CRE-decoy oligonucleotide can provide a powerful new means of combating cancers, viral diseases, and other pathological conditions by regulating the expression of cAMP-responsive genes.