Spatial organization of chromatin domains and compartments in single chromosomes.
Spatial organization of chromatin domains and compartments in single chromosomes.
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DOI：
10.1126/science.aaf8084
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发表时间：
2016-08-05
期刊：
影响因子：
--
通讯作者：
Zhuang X
中科院分区：
文献类型：
--
作者：
Wang S;Su JH;Beliveau BJ;Bintu B;Moffitt JR;Wu CT;Zhuang X
染色质的空间组织严重影响基因组功能。最近的染色体构象捕获研究揭示了拓扑相关结构域（TADs）作为染色质组织的保守特征，但TADs在单个染色体中的空间组织方式仍然未知。在这里，我们开发了一种成像方法映射的空间位置的许多基因组区域沿着个别染色体和跟踪的位置TADs在人类间期常染色体和X染色体。我们观察到，染色体折叠偏离理想的分形球模型在大的长度尺度和TADs在很大程度上被组织成两个隔间空间排列在一个极化的方式在个别染色体。活跃和不活跃的X染色体采用不同的折叠和区室化构型。这些结果表明，染色质结构域的空间组织可以改变调节。
The spatial organization of chromatin critically impacts genome function. Recent chromosome-conformation-capture studies have revealed topologically-associating domains (TADs) as a conserved feature of chromatin organization, but how TADs are spatially organized in individual chromosomes remains unknown. Here we developed an imaging method for mapping the spatial positions of numerous genomic regions along individual chromosomes and traced the positions of TADs in human interphase autosomes and X chromosomes. We observed that chromosome folding deviates from the ideal fractal-globule model at large length scales and that TADs are largely organized into two compartments spatially arranged in a polarized fashion in individual chromosomes. Active and inactive X chromosomes adopt different folding and compartmentalization configurations. These results suggest that the spatial organization of chromatin domains can change in response to regulation.