Evaluation of nanoparticle tracking analysis for total virus particle determination
Evaluation of nanoparticle tracking analysis for total virus particle determination
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DOI：
10.1186/1743-422x-9-265
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发表时间：
2012-11-12
期刊：
影响因子：
4.8
通讯作者：
Peterka, Matjaz
中科院分区：
文献类型：
--
作者：
Kramberger, Petra;Ciringer, Mateja;Peterka, Matjaz
采用纳米颗粒跟踪分析（NTA）软件对NanoSight LM10进行乳胶颗粒、腺病毒5和流感病毒的定量分析。通过连续几天测量同一样品来确定日间变化，并通过使用已知的主题颗粒浓度来证明该方法的准确性。在CIM整体柱上纯化腺病毒和流感病毒后，NTA分析也用于定量层析组分。将NTA结果与血凝（HA）和终点稀释法进行比较和评估，分别测定总病毒数和感染病毒颗粒数。结果表明，纳米颗粒跟踪分析是一种快速估计不同样品中病毒浓度的方法。此外，它还可以更好地了解样本状态，以及病毒聚集的水平。
The NanoSight LM10 with Nanoparticle tracking analysis (NTA) software was evaluated for the quantification of latex particles, adenovirus 5, and influenza virus. The inter-day variability was determined by measuring the same sample over several consecutive days and the method's accuracy was demonstrated by using known concentrations of the subject particles. NTA analysis was also used to quantify chromatographic fractions of adenovirus and influenza virus after purification on a CIM monolithic column. NTA results were compared and evaluated against hemagglutination (HA) and end point dilution assay, determining total and infection virus particle number, respectively. The results demonstrated that nanoparticle tracking analysis is a method for fast estimation of virus concentration in different samples. In addition, it can provide a better insight into the sample status, regarding the level of virus aggregation.