Plasmodium 6-cysteine proteins determine the commitment of sporozoites to liver-infection
Plasmodium 6-cysteine proteins determine the commitment of sporozoites to liver-infection
复制标题
疟原虫 6-半胱氨酸蛋白决定子孢子对肝脏感染的影响
DOI：
10.1016/j.parint.2022.102700
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发表时间：
2023
影响因子：
1.9
通讯作者：
Nishi Tsubasa
中科院分区：
文献类型：
--
作者：
Yuda Masao;Kaneko Izumi;Murata Yuho;Iwanaga Shiroh;Okubo Kazuhiro;Nishi Tsubasa
疟原虫子孢子从蚊子叮咬释放到肝脏的部位传播很长一段路，在那里它们感染肝细胞并发育成红细胞侵入形式。这种感染的成功取决于子孢子正确识别肝细胞作为靶标并将其行为从迁移改变为感染的能力。然而，这种变化是如何实现的仍然不完全清楚。在本文中，我们报告，6-半胱氨酸蛋白家族成员表达的子孢子，包括B 9负责这种能力。使用B 9和SPECT 2的双重敲除对寄生虫进行的实验表明，寄生虫缺乏停止迁移的能力，B 9和SPECT 2是子孢子迁移通过肝细胞所必需的。这一发现表明，这些寄生虫蛋白和肝细胞特异性细胞表面配体之间的相互作用介导子孢子正确识别肝细胞，这是疟疾传播到人类的重要步骤。
Plasmodium sporozoites travel a long way from the site where they are released by a mosquito bite to the liver, where they infect hepatocytes and develop into erythrocyte-invasive forms. The success of this infection depends on the ability of the sporozoites to correctly recognize the hepatocyte as a target and change their behavior from migration to infection. However, how this change is accomplished remains incompletely understood. In this paper, we report that 6-cysteine protein family members expressed in sporozoites including B9 are responsible for this ability. Experiments on parasites using double knockouts ofB9andSPECT2, which is essential for sporozoite to migrate through the hepatocyte, showed that the parasites lacked the capacity to stop migration. This finding suggests that interactions between these parasite proteins and hepatocyte-specific cell surface ligands mediate correct recognition of hepatocytes by sporozoites, which is an essential step in malaria transmission to humans.