P0 mRNA expression in cultures of Schwann cells and neurons that lack basal lamina and myelin.
P0 mRNA expression in cultures of Schwann cells and neurons that lack basal lamina and myelin.
复制标题
缺少基底层和髓磷脂的雪旺细胞和神经元培养物中 P0 mRNA 的表达。
DOI：
10.1002/jnr.490270206
复制
发表时间：
1990
影响因子：
4.2
通讯作者：
Brown,DT
中科院分区：
文献类型：
--
作者：
Brunden,KR;Brown,DT
周围神经系统的雪旺细胞依赖于轴突和基底层两者的存在来实现髓鞘形成表型。此外，轴突影响的去除导致髓鞘形成的停止和许旺细胞髓鞘蛋白表达的下调。在这里，我们研究是否轴突和基底层所需的雪旺细胞的主要髓鞘糖蛋白，宝编码的mRNA的表达。从15天胚胎大鼠幼崽的背根神经节获得的雪旺细胞和神经元的培养物在允许或禁止基底层和髓鞘形成的条件下在体外生长长达20天。测定这些培养物的Po表达。使用S1核酸酶保护试验检测mRNA。体外培养20天后，未组装基底层和不能形成髓鞘的培养物表达Po。mRNA的水平，这是在相同的年龄，髓鞘文化中看到的。无论是髓鞘和nonmyelinating文化表现出明显的增加PomRNA相比，开始胚胎背根神经节雪旺细胞。后者有一个低的，但可检测到的，这种髓鞘糖蛋白的mRNA水平。缺乏基底层和髓磷脂的培养物在培养11 - 15天时显示PomRNA的明显增加。这种表达的增加取决于神经元/神经突的存在，因为在神经元耗尽的培养物中生长的许旺细胞表达很少（如果有的话）PomRNA。对比阿宝的水平。mRNA，非髓鞘形成培养物具有显著较低的Po量。糖蛋白比组装髓磷脂的培养物更容易。这表明非髓鞘形成的雪旺细胞在翻译和/或翻译后水平调节这种糖蛋白的水平。这里提出的数据表明，髓鞘蛋白mRNA的表达和髓鞘组装许旺细胞是可分离的事件，与前者取决于一个或多个神经元/轴突因子。
Schwann cells of the peripheral nervous system depend on the presence of both axons and basal lamina to achieve a myelinating phenotype. Furthermore, removal of axonal influence results in the cessation of myelination and down‐regulation of myelin protein expression by Schwann cells. Here we examine whether both axons and basal lamina are required by Schwann cells for the expression of mRNA encoding the major myelin glycoprotein, Po. Cultures of Schwann cells and neurons obtained from dorsal root ganglia of 15 day embryonic rat pups were grown for up to 20 days in vitro under conditions that either allowed or prohibited basal lamina and myelin formation. These cultures were assayed for the expression of Po. mRNA by using an S1 nuclease‐protection assay. After 20 days in vitro, the cultures that did not assemble basal lamina and that were incapable of myelin formation expressed Po. mRNA at a level which was comparable to that seen in identically aged, myelinating cultures. Both the myelinating and nonmyelinating cultures demonstrated an appreciable increase in PomRNA when compared to the starting embryonic dorsal root ganglia Schwann cells. The latter had a low, but detectable, level of mRNA for this myelin glycoprotein. The cultures that were devoid of basal lamina and myelin showed a clear increase in PomRNA by 11–15 days in culture. This increase in expression depended on the presence of neurons/neurites, since Schwann cells which were grown in neuron‐depleted cultures expressed little, if any, PomRNA. In contrast to the levels of Po. mRNA, the nonmyelinating cultures had a significantly lower amount of Po. glycoprotein than did the cultures which assemble myelin. This suggests that the nonmyelinating Schwann cells regulate the level of this glycoprotein at the translational and/or the posttranslational level. The data presented here suggest that myelin protein mRNA expression and myelin assembly by Schwann cells are separable events, with the former depending on one or more neuronal/axonal factors.