Measurement of PDT-induced hypoxia in Dunning prostate tumors by iodine-123-iodoazomycin arabinoside.
Measurement of PDT-induced hypoxia in Dunning prostate tumors by iodine-123-iodoazomycin arabinoside.
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发表时间：
1993-03
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通讯作者：
Ronald B. Moore;Chapman Jd;J. Mercer;R. H. Mannan;L. Wiebe;A. Mcewan;M. McPhee
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作者：
Ronald B. Moore;Chapman Jd;J. Mercer;R. H. Mannan;L. Wiebe;A. Mcewan;M. McPhee
光动力疗法(PDT)在实体瘤中产生血管损伤，导致继发性缺血和肿瘤细胞缺氧死亡。用新的低氧标志物--123I-碘氮霉素阿拉伯糖苷(IAZA)研究了未治疗和PDT治疗的Dunning R3327-AT前列腺肿瘤在Fischer X哥本哈根大鼠体内的氧合状态。麻醉荷瘤大鼠平面闪烁成像的定性和定量数据显示，123I-IAZA在PDT治疗的肿瘤中的保留率增加。用99mTc-六亚甲基丙胺肟(HM-PAO)测定同一肿瘤的血流灌注。感兴趣区分析显示，~(123)I-IAZA测量的肿瘤乏氧与99mTC-HMPAO测量的肿瘤血流灌注呈负相关(相关系数r=-0.72)。一个大肿瘤2 mm冰冻切片的平面图像显示，123I-IAZA选择性地保留在接受PDT治疗的区域。这种和其他标记了~(123)I的阿奇霉素核苷在非侵入性监测肿瘤氧合状态方面有希望，特别是用于监测间质PDT治疗的有效性，在这种治疗中，血流阻断是肿瘤反应的主要机制。
Photodynamic therapy (PDT) is known to produce vascular damage in solid tumors resulting in secondary ischemia and tumor cell death from hypoxia. The oxygenation status of both non-treated and PDT-treated Dunning R3327-AT prostate tumors growing in Fischer X Copenhagen rats was investigated with the novel hypoxic marker, 123I-iodoazomycin arabinoside (IAZA). Both qualitative and quantitative data from planar scintigraphy of anesthetized tumor-bearing rats showed increased retention of 123I-IAZA in tumors treated with PDT. Tumor perfusion in the same tumors was measured with 99mTc-hexamethylpropyleneamine oxime (HM-PAO). Region of interest analyses revealed an inverse correlation between tumor hypoxia measured by 123I-IAZA and tumor perfusion as measured by 99mTc-HMPAO (coefficient of correlation, r = -0.72). Planar images of 2-mm frozen sections from a large tumor showed 123I-IAZA selectively retained in the region that had been treated with PDT. This and other iodinated azomycin nucleosides, labeled with 123I, show promise for monitoring tumor oxygenation status non-invasively and, in particular, for monitoring the effectiveness of interstitial PDT treatments where perfusion shutdown is a major mechanism of tumor response.