Erythrocyte damage of crucian carp (Carassius auratus) caused by microcystin-LR: in vitro study
Erythrocyte damage of crucian carp (Carassius auratus) caused by microcystin-LR: in vitro study
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DOI：
10.1007/s10695-011-9572-2
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发表时间：
2012-01
影响因子：
2.9
通讯作者：
Wenshan Zhou;Hualei Liang;Xuezhen Zhang
中科院分区：
文献类型：
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作者：
Wenshan Zhou;Hualei Liang;Xuezhen Zhang
鱼类在体内暴露于微囊藻毒素后会发生贫血和低血容量性休克。然而，除了贫血和低血压的体内原因外，体外研究暴露于MC的鱼红细胞是必要的。为了更好地了解微囊藻毒素的血液学毒性，本研究的主要目的是研究微囊藻毒素对鱼类红细胞的毒性作用。将鲫鱼红细胞与浓度为0、1、10、100和1,000 nM的微囊藻毒素-LR（MC-LR）进行体外孵育。MC-LR处理组脂质过氧化物水平显著升高。暴露于MC-LR后，Glucoside降低。MC-LR暴露后，超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽-S-转移酶等抗氧化酶活性显著升高。溶血作用明显增强，乙酰胆碱酯酶、Na+-K+-ATP酶和Ca ~（2+）-Mg ~（2+）-ATP酶活性明显降低。此外，在血涂片中观察到聚集和锯齿状红细胞的病理学改变。研究结果表明，红细胞的损伤也应该是贫血和水肿性休克甚至死亡的原因。
Fish suffer from anemia and hypovolemic hypotensive shock after in vivo exposure with microcystins. However, except for in vivo causes for anemia and hypotension, an in vitro study of fish erythrocytes exposed to MC is necessary. For a better understanding of hematology toxicity of MC, the main aim of the present study was to investigate the toxic effects of microcystin on fish erythrocytes in vitro. Crucian carp erythrocytes were incubated in vitro with microcystin-LR (MC-LR) at doses of 0, 1, 10, 100 and 1,000 nM. The level of lipid peroxidate significantly increased in MC-LR treatment groups. Glutathione decreased after exposure to MC-LR. The activities of antioxidative enzymes, including superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase, were significantly increased after exposure with MC-LR. The hemolysis was significantly increased, while the activities of acetylcholinesterase, Na+–K+-ATPase and Ca2+–Mg2+-ATPase were significantly decreased. In addition, pathological alterations in agglomerated and jagged erythrocytes were observed in blood smears. The findings indicate that damages to erythrocytes should also be responsible for anemia and hypotensive shock or even death.