Motif-based endomembrane trafficking
Motif-based endomembrane trafficking
复制标题
DOI：
10.1093/plphys/kiab077
复制
发表时间：
2021-02-19
期刊：
影响因子：
7.4
通讯作者：
Van Damme，Daniel
中科院分区：
文献类型：
--
作者：
Arora，Deepanksha;Van Damme，Daniel
膜内运输，允许蛋白质和脂类在不同的膜间隔之间流动，主要通过囊泡介导的运输发生。用于运输的跨膜蛋白通过供体膜的波动被浓缩到囊泡或载体中。这之后是囊泡破裂，去涂层，最后在靶膜上融合。真核细胞内有三条主要的转运途径：顺行、逆行和内吞。每条途径都涉及一套独特的机械和外壳蛋白，它们将跨膜蛋白及其相关的脂类包装到特定的载体中。接头和辅酶复合体是顺行转运和内吞作用的主要促进器。这些复合体识别要运输的跨膜货物，并招募有助于形成载体的外壳蛋白。这些复合体使用线性基序或翻译后修饰来识别货物，然后沿着贩运路径包装和运送。在这篇综述中，我们集中在不同的运输复合体，它们共享一个共同的进化分支，并讨论了关于它们使用的货物识别基序的最新知识。
Endomembrane trafficking, which allows proteins and lipids to flow between the different endomembrane compartments, largely occurs by vesicle-mediated transport. Transmembrane proteins intended for transport are concentrated into a vesicle or carrier by undulation of a donor membrane. This is followed by vesicle scission, uncoating, and finally, fusion at the target membrane. Three major trafficking pathways operate inside eukaryotic cells: anterograde, retrograde, and endocytic. Each pathway involves a unique set of machinery and coat proteins that pack the transmembrane proteins, along with their associated lipids, into specific carriers. Adaptor and coatomer complexes are major facilitators that function in anterograde transport and in endocytosis. These complexes recognize the transmembrane cargoes destined for transport and recruit the coat proteins that help form the carriers. These complexes use either linear motifs or posttranslational modifications to recognize the cargoes, which are then packaged and delivered along the trafficking pathways. In this review, we focus on the different trafficking complexes that share a common evolutionary branch in Arabidopsis (Arabidopsis thaliana), and we discuss up-to-date knowledge about the cargo recognition motifs they use.