Spindle dynamics during meiosis in Drosophila oocytes.
Spindle dynamics during meiosis in Drosophila oocytes.
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DOI：
10.1083/jcb.137.6.1321
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发表时间：
1997-06-16
期刊：
影响因子：
--
通讯作者：
Komma DJ
中科院分区：
文献类型：
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作者：
Endow SA;Komma DJ
果蝇成熟卵母细胞在减数分裂Ⅰ中期被阻滞。在排卵或受精激活后，卵母细胞经历了一系列以前没有直接可视化的快速变化。在这里，我们报告使用的Nonclaret分离（NCD）微管马达蛋白融合的绿色荧光蛋白（GFP），以监测活卵母细胞体外激活后的减数分裂纺锤体的变化。中期停滞卵母细胞的减数分裂纺锤体是相对稳定的，然而，体外激活卵母细胞的减数分裂纺锤体是高度动态的：纺锤体伸长，围绕其长轴旋转，并经历急性枢转运动以垂直于卵母细胞表面重新定向。许多卵母细胞自发地完成减数分裂，允许可视化从减数分裂I到II的进展。卵母细胞激活后纺锤体的运动提供了关于重新进入减数分裂和完成减数分裂分裂时纺锤体动态变化的新信息。还对与gfp融合的丧失功能的ncd等位基因突变体的活卵母细胞中的精子进行成像。活突变卵母细胞纺锤体缺陷的发生提供了新的见解Ncd功能的机制在纺锤体在减数分裂。
Mature oocytes of Drosophila are arrested in metaphase of meiosis I. Upon activation by ovulation or fertilization, oocytes undergo a series of rapid changes that have not been directly visualized previously. We report here the use of the Nonclaret disjunctional (Ncd) microtubule motor protein fused to the green fluorescent protein (GFP) to monitor changes in the meiotic spindle of live oocytes after activation in vitro. Meiotic spindles of metaphase-arrested oocytes are relatively stable, however, meiotic spindles of in vitro–activated oocytes are highly dynamic: the spindles elongate, rotate around their long axis, and undergo an acute pivoting movement to reorient perpendicular to the oocyte surface. Many oocytes spontaneously complete the meiotic divisions, permitting visualization of progression from meiosis I to II. The movements of the spindle after oocyte activation provide new information about the dynamic changes in the spindle that occur upon re-entry into meiosis and completion of the meiotic divisions. Spindles in live oocytes mutant for a lossof-function ncd allele fused to gfp were also imaged. The genesis of spindle defects in the live mutant oocytes provides new insights into the mechanism of Ncd function in the spindle during the meiotic divisions.