Subfatin is a Novel Adipokine and Unlike Meteorin in Adipose and Brain Expression
Subfatin is a Novel Adipokine and Unlike Meteorin in Adipose and Brain Expression
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Subfatin 是一种新型脂肪因子，与脂肪和大脑表达中的镍纹蛋白不同
DOI：
10.1111/cns.12219
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发表时间：
2014-04-01
影响因子：
5.5
通讯作者：
Miao, Chao-Yu
中科院分区：
文献类型：
--
作者：
Li, Zhi-Yong;Zheng, Si-Li;Miao, Chao-Yu
目的脂肪组织释放在代谢和心脑血管稳态中起重要作用的脂肪因子。本研究旨在利用热量限制模型发现新的脂肪因子。方法通过基因阵列和生物信息学分析捕获候选因子，制备重组蛋白和抗体进行验证。结果建立了包含208个基因的潜在分泌蛋白数据库，并在15个检测组织中鉴定出一种新的脂肪因子Subfatin，该因子在啮齿类动物和人类皮下脂肪中表达最高。分泌的哺乳动物亚脂肪素是一种糖基化蛋白。除脂滴外，亚脂肪素广泛分布于脂肪组织，在脂肪细胞和基质细胞中表达相似，但在巨噬细胞中的表达远低于脂肪细胞。亚脂肪素在热量限制大鼠的白色脂肪组织中下调，而在白色脂肪细胞分化和饮食诱导的肥胖小鼠的白色脂肪组织中显著上调。Subfatin在公共数据库中被注释为Meteorin-like (Metrnl)，这是Meteorin （Metrn，也称为胶质细胞分化调节剂）的类似转录本。与亚脂肪素的组织表达模式相反，Meteorin表现出脑特异性表达，在各种脂肪组织中都很少见。结论亚脂肪素是一种受脂肪形成和肥胖调控的新型脂肪因子，其组织分布与同源物Meteorin不同。
AimsAdipose tissue releases adipokines that play important roles in metabolic and cardio-cerebro-vascular homeostasis. This study was to discover novel adipokines using caloric restriction model.MethodsAdipokine candidates were captured by gene array and bioinformatics analysis and verified by preparation of recombinant protein and antibody.ResultsWe established a potential secreted protein database containing 208 genes and identified a novel adipokine, Subfatin, that was the highest expressed in subcutaneous fat of both rodents and humans among 15 detected tissues. The secreted mammalian Subfatin was a glycosylated protein. Subfatin was located diffusely throughout the adipose tissue except lipid droplets, with comparable expression between adipocytes and stromal cells, but much lower expression in macrophages than adipocytes. Subfatin was downregulated in white adipose tissue of caloric restriction rats, whereas dramatically upregulated during white adipocyte differentiation as well as in white adipose tissue of diet-induced obese mice. Subfatin was annotated as Meteorin-like (Metrnl) in public databases, a similar transcript of Meteorin (Metrn, also known as glial cell differentiation regulator). Meteorin displayed a brain-specific expression and was scarce in various adipose tissues, in contrast to the tissue expression patterns of Subfatin.ConclusionsSubfatin is a novel adipokine regulated by adipogenesis and obesity, with tissue distribution different from its homologue Meteorin.