Lack of expression of endometrial prolactin in early implantation failure: a pilot study
Lack of expression of endometrial prolactin in early implantation failure: a pilot study
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DOI：
10.1093/humrep/deh350
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发表时间：
2004-08-01
影响因子：
6.1
通讯作者：
Cetin, I
中科院分区：
文献类型：
--
作者：
Garzia, E;Borgato, S;Cetin, I
背景：动物模型和实验研究表明旁分泌催乳素（PRL）信号在蜕膜化和胚胎着床中的作用。我们研究了子宫内膜催乳素（e-PRL）和催乳素受体（PRL-R）在不明原因不孕（UI）和反复流产（RM）妇女子宫内膜中的表达。方法：患者（n=24）分为三组：RM，n=5; UI，n=11;对照组，n=8。在黄体晚期宫腔镜检查时采集子宫内膜样本。采用定性RT-PCR检测e-PRL和PRL-R转录本的存在。免疫组化法检测e-PRL的表达模式和表达部位。结果：3组子宫内膜组织中均检测到PRL-R mRNA。PRL基因表达检测在所有的对照样品中，只有在三个RM组的五个样品和四个UI组的11个样品。RT-PCR结果在很大程度上被免疫组化证实，研究组显示e-PRL表达缺陷。结论：在这项初步研究中，我们报告了一些不明原因不孕和反复流产患者在“着床窗”期子宫内膜催乳素表达的缺乏。这些数据，与那些在实验动物中获得的，表明子宫内膜PRL表达的缺乏参与生殖失败。
BACKGROUND: Animal models and experimental studies suggest a role for paracrine prolactin (PRL) signalling in decidualization and embryo implantation. We investigated the expression of endometrial prolactin (e-PRL) and prolactin receptor (PRL-R) in the endometrium of women affected by unexplained infertility (UI) and repeated miscarriages (RM). METHODS: Patients (n=24) were divided into three groups: RM, n=5; UI, n=11; controls, n=8. Endometrial samples were collected at the time of hysteroscopy in the late luteal phase. The presence of transcripts of e-PRL and PRL-R was investigated by qualitative RT-PCR. Pattern and site of expression of e-PRL were studied by immunohistochemistry. RESULTS: PRL-R mRNA was detected in all endometrial samples of the three groups. PRL gene expression was detected in all control samples, only in three of five samples of the RM group and in four of 11 samples of the UI group. RT-PCR results were largely confirmed by immunohistochemistry, study groups showing a defect of expression of e-PRL. CONCLUSIONS: In this pilot study we report a lack of expression of endometrial prolactin during the 'implantation window' in some patients affected by unexplained infertility and repeated miscarriages. These data, in association with those obtained in experimental animals, suggest that the lack of endometrial PRL expression is involved in reproduction failure.