Inhibitory Effect of Enterococcus faecium WB2000 on Volatile Sulfur Compound Production by Porphyromonas gingivalis.
Inhibitory Effect of Enterococcus faecium WB2000 on Volatile Sulfur Compound Production by Porphyromonas gingivalis.
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DOI：
10.1155/2016/8241681
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发表时间：
2016
影响因子：
2.1
通讯作者：
Hirofuji T
中科院分区：
文献类型：
--
作者：
Suzuki N;Higuchi T;Nakajima M;Fujimoto A;Morita H;Yoneda M;Hanioka T;Hirofuji T
口腔厌氧菌产生的挥发性硫化物(VSCs)是引起口腔异味的主要化合物。屎肠球菌WB2000是公认的一种抗菌斑益生菌。本研究评价了粪肠球菌WB2000对牙龈卟啉单胞菌产生VSC的影响，并探讨了其抑制口腔异味的机理。牙龈假单胞菌ATCC 33277在4种乳酸菌的存在下培养，其中包括粪肠球菌WB2000。随后，将牙龈假单胞菌ATCC 33277、W50、W83和两个临床分离株在有或没有屎埃希菌WB2000的情况下进行培养，并用气相色谱测定废培养上清液中血管干细胞的释放。与粪肠球菌混合培养6 h，牙龈假单胞菌ATCC 33277数量下降，且下降幅度大于与其他乳酸菌混合培养的下降速度。在与粪便肠杆菌WB2000混合培养时，5株牙龈假单胞菌的数量以相似的速度减少。与单独培养的牙龈假单胞菌相比，牙龈假单胞菌和粪肠球菌WB2000的废液中甲硫醇的浓度较低。因此，粪肠球菌WB2000可能通过抑制牙龈假单胞菌的生长和中和甲硫醇来减少口腔异味。
Volatile sulfur compounds (VSCs) produced by oral anaerobes are the major compounds responsible for oral malodor. Enterococcus faecium WB2000 is recognized as an antiplaque probiotic bacterium. In this study, the effect of E. faecium WB2000 on VSC production by Porphyromonas gingivalis was evaluated, and the mechanism of inhibition of oral malodor was investigated. P. gingivalis ATCC 33277 was cultured in the presence of four lactic acid bacteria, including E. faecium WB2000. Subsequently, P. gingivalis ATCC 33277, W50, W83, and two clinical isolates were cultured in the presence or absence of E. faecium WB2000, and the emission of VSCs from spent culture medium was measured by gas chromatography. The number of P. gingivalis ATCC 33277 in mixed culture with E. faecium WB2000 decreased at 6 h, and the rate of decrease was higher than that in mixed cultures with the other lactic acid bacteria. The numbers of five P. gingivalis strains decreased at similar rates in mixed culture with E. faecium WB2000. The concentration of methyl mercaptan was lower in spent culture medium from P. gingivalis and E. faecium WB2000 cultures compared with that from P. gingivalis alone. Therefore, E. faecium WB2000 may reduce oral malodor by inhibiting the growth of P. gingivalis and neutralizing methyl mercaptan.