A role for inositol hexakisphosphate in the maintenance of basal resistance to plant pathogens
A role for inositol hexakisphosphate in the maintenance of basal resistance to plant pathogens
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DOI：
10.1111/j.1365-313x.2008.03629.x
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发表时间：
2008-11-01
期刊：
影响因子：
7.2
通讯作者：
Hanke, David E.
中科院分区：
文献类型：
--
作者：
Murphy, Alex M.;Otto, Bettina;Hanke, David E.
植酸（myo-inositol hexakisphosphate，InsP（6））是植物体内重要的磷酸盐贮藏和信号分子。然而，正在开发低植酸植物，以尽量减少膳食InsP（6）的负面健康影响和动物粪便中未消化InsP（6）造成的污染。在组成型表达肌醇3-磷酸合酶（IPS，催化InsP（6）生物合成的第一步）或大肠杆菌多磷酸激酶的反义基因序列的转基因马铃薯植物中，InsP（6）水平降低。这些植物对无毒病原体马铃薯Y病毒和毒性病原体烟草花叶病毒（TMV）的抗性较低。在拟南芥中，催化InsP（6）生物合成最后一步的酶（InsP（5）2-激酶）的基因突变也降低了InsP（6）水平，并增强了对TMV以及细菌病原体假单胞菌的强毒和无毒菌株的敏感性。拟南芥（Arabidopsis thaliana）有三个IPS基因（AtIPS 1 -3）。突变体atips 2植物中InsP（6）被耗尽，并且对TMV、芜菁花叶病毒、黄瓜花叶病毒和花椰菜花叶病毒以及真菌灰葡萄孢（Botrytis cinerea）和P.突变体atips 2和atipk 1植物是hypersesceptible感染的植物不能积累水杨酸（SA），但他们的易感性增加是由于SA水平降低。相反，InsP（6）也被耗尽的突变体atips 1植物在对病原体的抗性方面没有受到损害，这表明InsP（6）的特定库调节对植物病原体的防御。
Phytic acid (myo-inositol hexakisphosphate, InsP(6)) is an important phosphate store and signal molecule in plants. However, low-phytate plants are being developed to minimize the negative health effects of dietary InsP(6) and pollution caused by undigested InsP(6) in animal waste. InsP(6) levels were diminished in transgenic potato plants constitutively expressing an antisense gene sequence for myo-inositol 3-phosphate synthase (IPS, catalysing the first step in InsP(6) biosynthesis) or Escherichia coli polyphosphate kinase. These plants were less resistant to the avirulent pathogen potato virus Y and the virulent pathogen tobacco mosaic virus (TMV). In Arabidopsis thaliana, mutation of the gene for the enzyme catalysing the final step of InsP(6) biosynthesis (InsP(5) 2-kinase) also diminished InsP(6) levels and enhanced susceptibility to TMV and to virulent and avirulent strains of the bacterial pathogen Pseudomonas syringae. Arabidopsis thaliana has three IPS genes (AtIPS1-3). Mutant atips2 plants were depleted in InsP(6) and were hypersusceptible to TMV, turnip mosaic virus, cucumber mosaic virus and cauliflower mosaic virus as well as to the fungus Botrytis cinerea and to P. syringae. Mutant atips2 and atipk1 plants were as hypersusceptible to infection as plants unable to accumulate salicylic acid (SA) but their increased susceptibility was not due to reduced levels of SA. In contrast, mutant atips1 plants, which were also depleted in InsP(6), were not compromised in resistance to pathogens, suggesting that a specific pool of InsP(6) regulates defence against phytopathogens.