Effect of glycocalyx on shear-dependent albumin uptake in endothelial cells
Effect of glycocalyx on shear-dependent albumin uptake in endothelial cells
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DOI：
10.1152/ajpheart.00808.2003
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发表时间：
2004-11-01
影响因子：
4.8
通讯作者：
Tanishita, K
中科院分区：
文献类型：
--
作者：
Ueda, A;Shimomura, M;Tanishita, K
内皮细胞表面的糖萼层是细胞摄取大分子（如低密度脂蛋白和白蛋白）的界面屏障。因此，大分子的剪切依赖性摄取可能支配糖萼层的功能。因此，我们研究了糖萼对内皮细胞剪切依赖性摄取大分子的影响。牛主动脉内皮细胞暴露于0.5 ~ 3.0Pa的剪切应力刺激48 h。用激光共聚焦显微镜观察细胞对白蛋白的摄取情况，用电子显微镜观察糖萼的显微结构。与静态条件下（无剪切应力刺激）内皮细胞的摄取相比，1.0 Pa剪切应力下的白蛋白摄取增加了16%，3.0 Pa剪切应力下的白蛋白摄取减少了27%。与静态条件相比，在3.0 Pa的剪切应力下，糖萼层厚度增加了70%，糖萼电荷增加了80%。在3.0 Pa的剪切应力下，中性（不带电）糖萼层的细胞的白蛋白摄取几乎是带电层细胞的两倍。这些研究结果表明，糖萼影响白蛋白的摄取在较高的剪切应力和糖萼的性质（厚度和电荷水平）参与剪切依赖性白蛋白的摄取过程。
The glycocalyx layer on the surface of an endothelial cell is an interface barrier for uptake of macromolecules, such as low-density lipoprotein and albumin, in the cell. The shear-dependent uptake of macromolecules thus might govern the function of the glycocalyx layer. We therefore studied the effect of glycocalyx on the shear-dependent uptake of macromolecules into endothelial cells. Bovine aorta endothelial cells were exposed to shear stress stimulus ranging from 0.5 to 3.0 Pa for 48 h. The albumin uptake into the cells was then measured using confocal laser scanning microscopy, and the microstructure of glycocalyx was observed using electron microscopy. Compared with the uptake into endothelial cells under static conditions ( no shear stress stimulus), the albumin uptake at a shear stress of 1.0 Pa increased by 16% and at 3.0 Pa decreased by 27%. Compared with static conditions, the thickness of the glycocalyx layer increased by 70% and the glycocalyx charge increased by 80% at a shear stress of 3.0 Pa. The albumin uptake at a shear stress of 3.0 Pa for cells with a neutralized ( no charge) glycocalyx layer was almost twice that of cells with charged layer. These findings indicate that glycocalyx influences the albumin uptake at higher shear stress and that glycocalyx properties ( thickness and charge level) are involved with the shear-dependent albumin uptake process.