A sulfide-sensor and a sulfane sulfur-sensor collectively regulate sulfur-oxidation for feather degradation by Bacillus licheniformis.

A sulfide-sensor and a sulfane sulfur-sensor collectively regulate sulfur-oxidation for feather degradation by Bacillus licheniformis.
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硫化物传感器和硫烷硫传感器共同调节地衣芽孢杆菌羽毛降解的硫氧化

DOI:
10.1038/s42003-023-04538-2
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发表时间:
2023-02-10
影响因子:
5.9
通讯作者:
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中科院分区:
生物学2区
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地衣芽孢杆菌MW3降解鸟类羽毛。羽毛角蛋白含有丰富的半胱氨酸,代谢后会产生有害的硫化物和亚硫酸盐。对生长在羽毛上的地衣芽孢杆菌MW3的挑战是给它们解毒。我们在地衣芽孢杆菌MW3中发现了一个基因簇来处理这些毒性。该基因簇包含11个基因,第1个基因yrkD编码抑制因子,第8和第9个基因nreB和nreC编码双组分调控系统,第10和第11个基因编码硫化:醌还原酶(SQR)和过硫氧化酶(PDO)。SQR和PDO共同将硫化物和亚硫酸盐硫氧化为亚硫酸盐。YrkD通过感知硫酸盐硫来抑制11个基因。NreBC系统检测到硫化物并进一步扩增sqr和pdo的转录。这两个调控系统协同控制了细菌在羽毛上生长所需的基因簇的表达。该研究结果强调了在羽毛降解过程中去除硫化物和亚砜硫的必要性,并可能有助于羽毛废物和硫化物污染的生物修复。
Bacillus licheniformis MW3 degrades bird feathers. Feather keratin is rich in cysteine, which is metabolized to produce hazardous sulfide and sulfane sulfur. A challenge to B. licheniformis MW3 growing on feathers is to detoxify them. Here we identified a gene cluster in B. licheniformis MW3 to deal with these toxicity. The cluster contains 11 genes: the first gene yrkD encodes a repressor, the 8th and 9th genes nreB and nreC encode a two-component regulatory system, and the 10th and 11th genes encode sulfide: quinone reductase (SQR) and persulfide oxygenase (PDO). SQR and PDO collectively oxidize sulfide and sulfane sulfur to sulfite. YrkD sensed sulfane sulfur to derepress the 11 genes. The NreBC system sensed sulfide and further amplified the transcription of sqr and pdo. The two regulatory systems synergistically controlled the expression of the gene cluster, which was required for the bacterium to grow on feather. The findings highlight the necessity of removing sulfide and sulfane sulfur during feather degradation and may help with bioremediation of feather waste and sulfide pollution.
转录调节剂中硫化物感应特异性的结构基础。
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发表时间: 2021-01
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DOI: 10.1128/jb.184.23.6624-6634.2002
发表时间: 2002-12-01
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Fedtke, I;Kamps, A;Götz, F
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Cyclic-di-GMP 通过 RsmA 和 RsmE 蛋白调节荧光假单胞菌 2P24 的群体感应系统和生物防治活性。
DOI: 10.1128/aem.02016-20
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