Significant allelic loss of ANX7 region (10q21) in hormone receptor negative breast carcinomas
Significant allelic loss of ANX7 region (10q21) in hormone receptor negative breast carcinomas
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DOI：
10.1016/j.canlet.2004.01.018
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发表时间：
2004-07-16
期刊：
影响因子：
9.7
通讯作者：
Srivastava, M
中科院分区：
文献类型：
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作者：
Leighton, X;Srikantan, V;Srivastava, M
在35%的前列腺肿瘤中发现了肿瘤抑制基因ANX 7-GTX基因10 q21区域的杂合性丢失（洛）。因此，这项研究的基本原理是，该基因也可能与乳腺发病机制有关。我们研究了10 q21区域微卫星的等位基因丢失，以及它们与ANX 7状态、雌激素受体（ER）状态、孕激素受体（PR）状态、Ki-67状态和病理表型的相关性，并在30例乳腺癌中进行了配对对照。通过PCR扩增DNA进行洛分析，使用10 q21区域的四个标记（AFMa 299 ya 5、AFM 220 xe 5、AFM 063 xc 5、AFM 200 wf 4）。在包含10对标本的第一组信息性肿瘤中，在10 q21区域的至少一个标记（AFM 220 xe 5标记接近ANX 7）中发现66%的洛。随后使用AFM 220 xe 5对20例癌进行了病理学参数的比较，结果显示有或无洛缺失与年龄差异有显著相关性（P = 0.04），ER（P = 0.05）、Ki-67（P = 0.04）和PR（P = 0.01）;发现肿瘤大小（P = 0.06）和组织学分级III（P = 0.14）有显著性趋势。这些结果表明，ANX 7基因或10 q21区域的其他基因可能与乳腺癌功能相关，可能影响与发育过程中不良预后相关的激素受体表达。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
Loss of heterozygosity (LOH) in the 10q21 region that harbors the tumor suppressor gene ANX7-GTPase gene have been found in 35% of prostate tumors. Therefore, the rationale for this study is that this gene could also be implicated in breast pathogenesis as well. We investigated allelic losses in microsatellites of the 10q21 region, and their correlations with ANX7 status, estrogen receptor (ER) status, progesterone receptor (PR) status, Ki-67 status and pathological phenotype in 30 breast carcinomas with matched control specimens. The LOH analysis was performed by amplifying DNA by PCR, using four markers of the 10q21 region (AFMa299ya5, AFM220xe5, AFM 063xc5, AFM200wf4). LOH in at least one marker of the 10q21 region (AFM220xe5 marker close to ANX7) was found in 66% of the first set of informative tumors containing 10 pairs of specimens. Subsequent comparison between 20 carcinomas using AFM220xe5, with and without LOH in terms of pathological parameters showed significant associations with differences in age (P = 0.04), ER (P = 0.05), Ki-67 (P = 0.04) and PR (P = 0.01); a trend toward significance was found for tumor size (P = 0.06) and histological grade III (P = 0.14). These results suggest that the ANX7 gene, or other genes of the 10q21 region, could be functionally related to breast cancer, probably influencing the hormone receptor expression associated with poor prognosis during development. (C) 2004 Elsevier Ireland Ltd. All rights reserved.