Switching metalloporphyrin binding specificity of a b -type cytochrome to fluorogenic zinc by design
Switching metalloporphyrin binding specificity of a b -type cytochrome to fluorogenic zinc by design
复制标题
通过设计将b型细胞色素的金属卟啉结合特异性切换为荧光锌
DOI：
10.1101/832923
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Bowen B
中科院分区：
文献类型：
--
作者：
Bowen B
金属卟啉在从生物学到纳米科学等领域发挥着重要作用。生物学使用一组狭窄的金属中心，主要由铁和镁组成。在这里，我们将细胞色素b562的金属卟啉特异性从铁（血红素）转换为荧光锌原卟啉IX（ZnPP）。通过计算引导的迭代设计过程，产生了对ZnPP具有接近总偏好的变体，其代表特异性的切换。新的变体大大增强（≥60倍）游离ZnPP在体外和体内的可忽略的水荧光。
Metalloporphyrins play important roles in areas ranging from biology to nanoscience. Biology uses a narrow set of metal centres comprising mainly of iron and magnesium. Here, we convert metalloporphyrin specificity of cytochromeb562from iron (haem) to fluorogenic zinc protoporphyrin IX (ZnPP). Through a computationally guided iterative design process, a variant with a near total preference for ZnPP was generated representing a switch in specificity. The new variant greatly enhanced (≥60 fold) the negligible aqueous fluorescence of free ZnPPin vitroandin vivo.