Adoptive Transfer of Serum Samples From Children With Invasive Staphylococcal Infection and Protection Against Staphylococcus aureus Sepsis.
Adoptive Transfer of Serum Samples From Children With Invasive Staphylococcal Infection and Protection Against Staphylococcus aureus Sepsis.
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侵袭性葡萄球菌感染儿童血清样本的过继转移和金黄色葡萄球菌脓毒症的预防。
DOI：
10.1093/infdis/jiaa482
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Thomsen,Isaac
中科院分区：
文献类型：
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作者：
Tsai,Chih-Ming;Soper,Nicole;Bennett,Monique;Fallon,JonathanK;Michell,AshlinR;Alter,Galit;Liu,GeorgeY;Thomsen,Isaac
成功的金黄色葡萄球菌疫苗仍然难以捉摸，该领域的一个争议是人类是否对感染产生保护性适应性免疫反应。我们开发了一种细菌挑战鼠试验，直接评估过继转移的人血清样品的保护能力。我们首先验证了该模型，表明肺炎球菌疫苗后的人类血清样品诱导小鼠有效清除肺炎链球菌。然后我们发现，人血清样本过继转移自患有侵袭性疾病的儿童。金黄色葡萄球菌感染在鼠模型中表现出对疾病的保护，一些样品提供了接近完全的保护。这些发现表明，人血清样品能够赋予人类在侵袭性葡萄球菌疾病期间产生的保护性适应性反应，从而允许在小鼠模型中研究保护性因子。鉴定最有效的血清样品中存在的保护因子将作为潜在的葡萄球菌疫苗候选物或被动治疗剂具有高度的兴趣。
A successfulStaphylococcus aureusvaccine remains elusive, and one controversy in the field is whether humans generate a protective adaptive immune response to infection. We developed a bacterial challenge murine assay that directly assesses the protective capacity of adoptively transferred human serum samples. We first validated the model by showing that postpneumococcal vaccine serum samples from humans induced effective clearance ofStreptococcus pneumoniaein mice. We then found that human serum samples adoptively transferred from children with invasiveS. aureusinfections exhibited protection from disease in a murine model, with some samples conferring near complete protection. These findings demonstrate that human serum samples are capable of conferring a protective adaptive response generated by humans during invasive staphylococcal disease, allowing for the study of protective factors in a murine model. Identification of the protective factors present in the most efficacious serum samples would be of high interest as potential staphylococcal vaccine candidates or passive therapeutics.