Bestatin analogue from Streptomyces neyagawaensis SL-387
Bestatin analogue from Streptomyces neyagawaensis SL-387
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DOI：
10.1271/bbb.60.898
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发表时间：
1996-05-01
影响因子：
1.6
通讯作者：
Kho, YH
中科院分区：
文献类型：
--
作者：
Chung, MC;Lee, HJ;Kho, YH
在含有DL-3-氨基-3-苯丙酸的化学介质中，从内氏链霉菌SL-387的培养滤液中获得了一种最佳抑素类似物(2S，3R)-3-amino-2-hydroxy-1-phenylbutanoyl-L-valine(AHPA-Val)，其对猪肾微粒体氨基肽酶N的抑制作用是贝斯汀的6倍(IC_(50)=1.2mU/ml)。对人转移性纤维肉瘤HT1080氨基肽酶N的抑制作用为Bestatin的4倍(5.6mU/ml)(IC50=20.7mU/ml)，据我们所知，这是有关微生物生产AHPA-Val的首次报道。
A bestatin analogue, (2S,3R)-3-amino-2-hydroxy-1-phenylbutanoyl-L-valine (AHPA-Val), from the culture filtrate of Streptomyces neyagawaensis SL-387 was obtained in a chemically defined medium containing DL-3-amino-3-phenylpropionic acid, AHPA-Val was 6 times (IC50 = 1.2 mu g/ml) as strong as bestatin (IC50 = 7.0 mu g/ml) against porcine kidney microsomal aminopeptidase N, and 4 times (5.6 mu g/ml) as strong as bestatin (IC50 = 20.7 mu g/ml) against aminopeptidase N of human metastatic fibrosarcoma HT1080, To the best of our knowledge, this is the first report on the microbial production of AHPA-Val.