A conserved noncoding intronic transcript at the mouse Dnm3 locus
A conserved noncoding intronic transcript at the mouse Dnm3 locus
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DOI：
10.1016/j.ygeno.2005.02.001
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发表时间：
2005-06-01
期刊：
影响因子：
4.4
通讯作者：
Tam, PPL
中科院分区：
文献类型：
--
作者：
Loebel, DAF;Tsoi, B;Tam, PPL
一个包含在小鼠DmO基因内含子内的6kb反义转录物（Dnm3os）击中。该反义转录物在脊椎动物物种之间高度保守，但似乎不编码蛋白质。我们发现，Dnm3和Dnm3os转录本在胚胎发生和成人组织中的表达是重叠的，除了Dnm3在成人脑和睾丸中表达最多，在胚胎中表达水平较低，而反义转录本在胚胎和妊娠子宫中表达最强烈，Dnm3和Dnm3os在Twist-null胚胎的鳃弓组织中均下调。这种非编码转录物的保守性和限制性表达模式表明，它可能在胚胎发育过程中发挥重要作用，并进一步表明Dnm3的调控与这种新的内含子反义转录物之间存在联系。(c) 2005爱思唯尔公司版权所有。
A 6-kb antisense transcript (Dnm3os) contained within an intron of the mouse DmO gene hit,.,, been identified in a screen forgeries that may be regulated by the basic helix-loop-helix transcription factor Twist during mouse development, The antisense transcript is highly conserved between vertebrate species, but does not appear to encode a protein. We show that expression of the Dnm3 and Dnm3os transcripts overlaps during embryogenesis and in adult tissues, except that Dnm3 is most highly expressed in adult brain and testis and expressed at lower levels in embryos, whereas the antisense transcript is most strongly expressed in embryos and gravid uterus, Both Dnm3 and Dnm3os are downregulated in branchial arch tissue of Twist-null embryos. The conservation and restricted expression pattern of this noncoding transcript suggest that it may perform an important function during embryonic development and further Suggest a connection between the regulation of Dnm3 and this novel intronic, antisense transcript. (c) 2005 Elsevier Inc. All rights reserved.