Implantation study of a novel hydroxyapatite/collagen (HAp/Col) composite into weight-bearing sites of dogs
Implantation study of a novel hydroxyapatite/collagen (HAp/Col) composite into weight-bearing sites of dogs
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DOI：
10.1002/jbm.10305
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发表时间：
2002-10-01
期刊：
影响因子：
--
通讯作者：
Shinomiya, K
中科院分区：
文献类型：
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作者：
Itoh, S;Kikuchi, M;Shinomiya, K
制备了一种羟基磷灰石/I型胶原(HAP/Col)复合材料，使羟基磷灰石纳米晶沿胶原分子取向。对这种新型生物材料植入负重区的生物相容性、骨传导活性以及作为rhBMP-2载体的有效性进行了检测。将直径15 mm、长20 mm、表面交联层含有重组人骨形态发生蛋白-2(0或400µg/ml)的HAP/Col种植体植入3只比格犬的胫骨缺损处，按Ilizarov方法固定。作为对照，两只比格犬的20个修女的骨缺损没有接受植入，允许狗使用伊利扎罗夫骨外固定器行走。12周后，每组取一只狗的标本。同时，在12周后取出接受重组人骨形态发生蛋白治疗的犬的Ilizarov固定器，之后开始完全负重。分别于18周、24周和24周后取出标本，对照组在24周后取出标本。骨密度的变化以及放射学和组织学结果表明，种植体能够诱导骨重建单位，并且由于早期骨痂和新骨形成的刺激，是重组人骨形成蛋白-2的良好载体。(C)2002年威利期刊公司。
A hydroxyapatite/type I collagen (HAp/Col) composite, aligning hydroxyapatite nanocrystals along collagen molecules, has been prepared. The biocompatibility, osteoconductive activity, and efficacy as a carrier of rhBMP-2 of this novel biomaterial implanted in the weight-bearing site have been examined. The HAp/Col implants (15 mm in diameter and 20 mm in length) with a surface cross-linked layer containing rhBMP-2 (0 or 400 mug/ml) were implanted into bone defects of tibiae in three beagle dogs and fixed according to the Ilizarov method. As a control, bone defects of 20 nun in two beagle dogs did not receive implants, and the dogs were allowed to walk using an Ilizarov extraskeletal fixator. The specimens were removed from one dog in each group after 12 weeks. Also, the Ilizarov fixators in the rhBMP-treated dogs were removed after 12 weeks, after which full weight bearing started. The specimens were further taken out after 18 and 24 weeks in the rhBMP-treated and non-rhBMP-treated dogs, and after 24 weeks in the control group. The change of bone mineral density, as well as radiological and histological findings, suggest that the implants are able to induce bone remodeling units and are a superior carrier of rhBMP-2 due to the stimulation of early callus and new bone formation. (C) 2002 Wiley Periodicals, Inc.