Characterization and expression pattern analysis of pheromone receptor-like genes in Winter MushroomFlammulina filiformis
Characterization and expression pattern analysis of pheromone receptor-like genes in Winter MushroomFlammulina filiformis
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冬菇金针菇信息素受体类基因的特征及表达模式分析
DOI：
10.1007/s00203-020-01990-0
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发表时间：
2020
影响因子：
2.8
通讯作者：
Wang Wei
中科院分区：
文献类型：
--
作者：
Meng Li;Chou Tiansheng;Jiang Siyuan;Wang Li;Zhu Mengjuan;Mukhtar Irum;Xie Baogui;Wang Wei
信息素受体样基因（Pheromone receptor-like genes，PRLG）属于G蛋白偶联受体（G protein-coupled receptors，GPCRs）家族，在真核细胞中与生物和非生物刺激物相互作用并将信号传递到细胞内下游通路。本研究对丝状金针菇PRLG基因的结构和表达模式进行了研究，通过序列比对分析发现丝状金针菇PRLG基因在不同菌株中具有保守的结构，只有少量的单核苷酸多态性（single nucleotide polymorphism，SNP）位点。在F.丝状菌这些基因含有2-5个内含子，但在供试菌株的编码蛋白序列中，内含子的相对位置并不相同。在PRLGs的肽段结构中发现了三种保守的基序，而FfSte3.s6只包含两种类型，表明其在进化和功能上存在差异。进一步采用实时荧光定量聚合酶链反应（qRT-PCR）分析了丝状镰刀菌子实体不同发育阶段各PRLG基因的表达模式。结果表明，PRLG基因在不同发育阶段的表达存在差异。丝状的FfSte 3. s1和FfSte 3. s2在菌丝期表达量最高。PRLG在子实体发育阶段抑制高表达。本研究表明PRLG基因可能是参与丝状真菌子实体发育的重要基因，但其在子实体发育中的具体功能途径有待进一步研究。
Pheromone receptor-like genes (PRLGs) belong to the G protein-coupled receptors (GPCRs) family that interacts with biotic and abiotic stimulants and transmits signals to intracellular downstream pathways in eukaryotic cells. In this study, we investigated the structure and expressions patterns ofPRLGsin Winter MushroomFlammulina filiformis.Based on the alignment analysis, the structure ofPRLGswas found conserved inF. filiformisstrains expect few single-nucleotide polymorphism (SNP) sites. SixPRLGswere found at five different unlinked loci, scattered in the genomes ofF. filiformisstrains. These genes contain 2–5 introns; however, the introns were not found in the same relative positions regarding the encoded protein sequences in tested strains ofF. filiformis.Three conserved motifs were identified in peptides structures ofPRLGs, however, FfSte3.s6 contained only two types, suggests its difference in evolution and function. We have further analyzed the expression patterns of eachPRLGsin different developmental stages of the fruiting body inF. filiformisby quantitative real-time polymerase chain reaction (qRT-PCR). The results exhibited expression variation ofPRLGsat different developmental stages of theF. filiformis. Especially,FfSte3.s1andFfSte3.s2exhibited maximum expression level in mycelia stage. OtherPRLGsexhibited high expression level in fruiting body stages. This study suggests thatPRLGscould be vital genes involving in fruiting body development inF. filiformis.However, further studies could be performed to reveal their specific functional pathways in the fruiting body development.