Increased Expression of IL-23 Receptor (IL-23R) in Vogt-Koyanagi-Harada (VKH) Disease.
Increased Expression of IL-23 Receptor (IL-23R) in Vogt-Koyanagi-Harada (VKH) Disease.
复制标题
Vogt-小柳-原田 (VKH) 病中 IL-23 受体 (IL-23R) 的表达增加。
DOI：
10.1080/02713683.2018.1485952
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发表时间：
2018
影响因子：
2
通讯作者：
Yang Peizeng
中科院分区：
文献类型：
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作者：
Wang Chaokui;Wang Luoziyi;Hu Jianping;Li Hong;Kijlstra Aize;Yang Peizeng
目的：Vogt-小柳-原田病(Vogt-Koyanagi-Harada，VKH)是一种以黑素细胞为靶细胞的T细胞介导的自身免疫性疾病。已有研究表明，IL-23受体(IL-23R)信号通路促进致病T辅助17细胞的产生。本研究旨在探讨IL-23R在VKH疾病中的可能作用。方法：将受试者分为活动期和非活动期VKH患者组和正常对照组。用流式细胞仪和实时定量聚合酶链式反应检测外周血单个核细胞(PBMC)中IL-23R水平。用VKH患者血清或对照组血清刺激PBMC，检测VKH患者血清对IL-23R表达的影响。结果：VKH活动期患者PBMC中IL-23R基因表达水平明显高于正常对照组。流式细胞仪分析显示活动期VKH患者PBMC中IL-23R蛋白水平升高。正常人PBMC与活动期VKH患者血清培养的IL-23R蛋白水平高于正常人血清培养的PBMC。VKH患者眼内炎症控制后，IL-23R基因表达恢复到正常水平。结论：IL-23R水平升高可能参与了VKH的发病过程。
Objective: Vogt–Koyanagi–Harada (VKH) disease is an autoimmune disease mediated by T cells that target melanocytes. It has been shown that IL-23 receptor (IL-23R) signaling promotes the generation of pathogenic T helper 17 cells. The aim of this study was designed to detect the possible role of IL-23R in VKH disease.Methods: Subjects were divided into an active and inactive VKH patient group and a normal control group. The IL-23R level in peripheral blood mononuclear cells (PBMCs) was measured by flow cytometry and real-time polymerase chain reaction. PBMCs were stimulated with serum from patients or controls to detect the influence of serum from VKH patients on IL-23R expression.Results: The IL-23R mRNA level was markedly increased in PBMCs from the active VKH patient group as compared to normal controls. Flow cytometry analysis showed that there was also an elevated IL-23R protein level in PBMCs in active VKH patients. The IL-23R protein level was higher in PBMCs obtained from healthy controls when they were cultured with serum from active VKH patient as compared to cell cultured with serum from normal controls. After the intraocular inflammation in VKH patients was controlled, the IL-23R gene expression returned back to normal levels.Conclusion: Our study suggests that an elevated IL-23R level may participate in the development of VKH disease.