Collagen zymography as a sensitive and specific technique for the determination of subpicogram levels of interstitial collagenase
Collagen zymography as a sensitive and specific technique for the determination of subpicogram levels of interstitial collagenase
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DOI：
10.1006/abio.1997.2318
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发表时间：
1998-01-15
影响因子：
2.9
通讯作者：
Pellat, B
中科院分区：
文献类型：
--
作者：
Gogly, B;Groult, N;Pellat, B
We describe the use of casting native collagen type I in SDS-polyacrylamide gel (collagen zymography) for the determination of interstitial collagenase. As with gelatin, the incorporation of collagen in the gels reduced protein migration and the need for making corrections for an accurate M-r evaluation. This method proved to be very sensitive: 0.1 pg of APMA-activated procollagenase could be detected, and specific levels of active gelatinase or stromelysin lower than 5 ng were inactive under our experimental conditions. It was used to demonstrate the increased expression of collagenase following treatment of human gingival fibroblasts with interleukin-1 beta; the amounts of enzyme quantified by either collagen zymography or immunodot blot assay are comparable. (C) 1998 Academic Press.
