Flicker-photometric electroretinogram estimates of L:M cone photoreceptor ratio in men with photopigment spectra derived from genetics
Flicker-photometric electroretinogram estimates of L:M cone photoreceptor ratio in men with photopigment spectra derived from genetics
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DOI：
10.1364/josaa.17.000499
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发表时间：
2000-03-01
影响因子：
1.9
通讯作者：
Neitz, J
中科院分区：
文献类型：
--
作者：
Carroll, J;McMahon, C;Neitz, J
利用闪烁光度视网膜电图（ERG）估计了长波长敏感(L)和中波长敏感(M)视锥细胞的相对比例。已经证明，光谱光度函数估算锥体比例的一个主要误差来源是已知的光色素λ (max)的变化[Vision Res. 38, 1961(1998)]。为了纠正这些错误，利用从每个受试者的光色素基因序列中推断出的个性化l -锥光谱灵敏度曲线，得出了锥体比例的估计。对于某些人来说，这种修正使估计的锥体比例与假设固定标准L锥体时获得的值有很大的不同。最大的差异发生在假设标准L色素时，估计有62%的L视锥细胞（L:M比1.6:1），但当使用他的个性化L视锥光谱时，估计有80%的L视锥细胞（L:RI比4:1）。通过ERG的反复测量，确定了相对L-to- m锥体贡献的个体估计，用%L锥体表示，通常在2%左右是可靠的。15名男性受试者的平均L:M比值估计为2:1 （67% L锥）。以前，从光度法获得的L:M比值有很大范围的个体变异性。一个尚未解决的问题是，有多少范围可能归因于错误。在这方面，已采取措施显著减少测量误差。尽管如此，很大范围的个体差异仍然存在。估计的L:M比值在0.6:1到12:1之间（40% L到92% L）. (C) 2000光学学会美国[S0740-3232(00)01903-7]。
Relative proportions of long-wavelength-sensitive (L) to middle-wavelength-sensitive (M) cones were estimated by use of the flicker-photometric electroretinogram (ERG). It has been demonstrated that a major source of error in estimates of cone proportions from spectral luminosity functions is the known variation in the lambda(max) of the photopigments [Vision Res. 38, 1961 (1998)]. To correct for these errors, estimates of cone proportions were derived by use of individualized L-cone spectral sensitivity curves deduced from photopigment gene sequences from each subject. For some individuals this correction made a large difference in the estimated cone proportions compared with the value obtained when a fixed standard L cone was assumed. The largest discrepancy occurred in a man estimated to have 62% L cones (L:M ratio 1.6:1) when a standard L pigment was assumed but a value of 80% L cones (L:RI ratio 4:1) when his individualized L-cone spectrum was used. From repeated measurements made with the ERG, it was determined that individual estimates of the relative L-to-M cone contributions, expressed as %L cones, are usually reliable within similar to 2%. The average L:M ratio for 15 male subjects was estimated at 2:1 (67% L cones). Previously, a large range of individual variability was reported for L:M ratios obtained from photometry. An unresolved issue concerns how much of the range might be attributed to error. Here efforts have been taken to markedly reduce measurement error. Nonetheless, a large range of individual differences persists. Estimated L:M ratios for individuals ranged from 0.6:1 to 12:1 (40% L to 92% L). (C) 2000 Optical Society of America [S0740-3232(00)01903-7].