Frequent asymptomatic infection with tobacco ringspot virus on melon fruit
Frequent asymptomatic infection with tobacco ringspot virus on melon fruit
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DOI：
10.1016/j.virusres.2020.198266
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发表时间：
2021-02-01
期刊：
影响因子：
5
通讯作者：
Fukuhara, Toshiyuki
中科院分区：
文献类型：
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作者：
Tabara, Midori;Nagashima, Yukihiro;Fukuhara, Toshiyuki
甜瓜是世界上最受欢迎的水果之一，并已被培育成各种品种。使用健康甜瓜果实进行RNA测序以确定品种之间基因表达的差异。出乎意料的RNA-seq结果显示，病毒以高频率无症状地感染水果（检查的21个水果中有16个被感染），并且与宿主转录物相比，病毒转录物高度积累（总读取的15%-75%）。核苷酸序列分析和系统发育分析表明，在甜瓜果实中发现了10多个新的烟草环斑病毒（TRSV）分离物。通过免疫印迹和RT PCR分析证实甜瓜果实上存在TRSV无症状感染。大量的TRSV分离物产生并维持在甜瓜田地中，这可能是由于它们的无症状感染。该TRSV甜瓜分离物感染本氏烟草（Nicotiana benthamiana）植物，具有矮化和黄化症状。这是第一次在食用瓜类水果中频繁和无症状地感染TRSV的报告。
Melon is one of the most popular fruits worldwide and has been bred into various cultivars. RNA sequencing using healthy melon fruit was performed to determine differences in gene expression among cultivars. Unexpected RNA-seq results revealed that viruses asymptomatically infected fruits at a high frequency (16 of 21 fruits examined were infected) and that viral transcripts highly accumulated in comparison with host transcripts (15 %-75 % of total reads). Their nucleotide sequences and phylogenetic analyses indicated that more than 10 novel isolates of tobacco ringspot virus (TRSV) were found in melon fruits. Asymptomatic infection with TRSV on melon fruits was confirmed by both immunoblot and RT PCR analyses. Numerous isolates of TRSV generated and maintained in melon fields, and this is likely due to their asymptomatic infections. This TRSV melon isolate infected Nicotiana benthamiana plants with stunting and yellowing symptoms. This is the first report of frequent and asymptomatic infection of TRSV in consumable melon fruits.