Rapid isolation and identification of bacteriophage T4-encoded modifications of Escherichia coli RNA polymerase:: A generic method to study bacteriophage/host interactions
Rapid isolation and identification of bacteriophage T4-encoded modifications of Escherichia coli RNA polymerase:: A generic method to study bacteriophage/host interactions
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DOI：
10.1021/pr070451j
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发表时间：
2008-03-01
影响因子：
4.4
通讯作者：
Darst, Seth A.
中科院分区：
文献类型：
--
作者：
Westblade, Lars F.;Minakhin, Leonid;Darst, Seth A.
噬菌体是感染细菌细胞的细菌病毒，它们开发出巧妙的机制来修饰细菌 RNA 聚合酶。使用快速、特异性、单步亲和分离程序从噬菌体 T4 感染的细胞中纯化大肠杆菌 RNA 聚合酶，我们鉴定了宿主 RNA 聚合酶的噬菌体 T4 依赖性修饰。我们建议这种方法广泛适用于鉴定宿主合成机制的噬菌体依赖性改变。
Bacteriophages are bacterial viruses that infect bacterial cells, and they have developed ingenious mechanisms to modify the bacterial RNA polymerase. Using a rapid, specific, single-step affinity isolation procedure to purify Escherichia coli RNA polymerase from bacteriophage T4-infected cells, we have identified bacteriophage T4-dependent modifications of the host RNA polymerase. We suggest that this methodology is broadly applicable for the identification of bacteriophage-dependent alterations of the host synthesis machinery.