HUMAN HISTOCOMPATIBILITY LEUKOCYTE ANTIGEN-DR AND HEAT-SHOCK PROTEIN-70 EXPRESSION IN EYE MUSCLE-TISSUE IN THYROID-ASSOCIATED OPHTHALMOPATHY
HUMAN HISTOCOMPATIBILITY LEUKOCYTE ANTIGEN-DR AND HEAT-SHOCK PROTEIN-70 EXPRESSION IN EYE MUSCLE-TISSUE IN THYROID-ASSOCIATED OPHTHALMOPATHY
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DOI：
10.1210/jc.80.2.685
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发表时间：
1995-02-01
影响因子：
5.8
通讯作者：
NONAKA, K
中科院分区：
文献类型：
--
作者：
HIROMATSU, Y;TANAKA, K;NONAKA, K
为了研究甲状腺相关眼病（TAO）患者眼眶组织中人类白细胞抗原（HLA）-DR和热休克蛋白70（HSP-70）的表达和定位，我们使用抗HLA-DR和抗HSP-70单克隆抗体以及链霉亲和素-生物素-过氧化物酶检测系统进行了免疫组织化学研究。在手术中从 38 名 TAO 患者和 8 名对照受试者身上获取眼肌组织。在 3 名未治疗患者中的 2 名和 35 名接受眼眶放疗或皮质类固醇治疗的患者中的 2 名患者的眼眶组织中证实了眼肌细胞上的 HLA-DR 表达，在所有这些患者中也证实了淋巴细胞浸润。在所研究的 8 名正常对照的眼肌细胞中未检测到 HLA-DR。 HLA-DR 在几乎所有 TAO 患者和所有 8 名对照受试者的内皮细胞和间质细胞上表达。在 31 名 TAO 患者（包括所有 3 名未经治疗的患者和 3 名对照患者）的眼肌细胞中检测到 HSP-70。尽管 HSP-70 表达程度与眼病的严重程度无关，但短期眼病患者 (83%) 的眼肌细胞中 HSP-70 的显着表达比病程较长的患者 (33%) 更常见。这些结果支持这样的观点，即眼肌纤维是 TAO 特征的眼眶自身免疫反应的重要靶标。
To investigate the expression and localization of human leukocyte antigen (HLA)-DR and heat shock protein-70 (HSP-70) in orbital tissue from patients with thyroid-associated ophthalmopathy (TAO), we carried out an immunohistochemical study using anti-HLA-DR and anti-HSP-70 monoclonal antibodies and a streptavidin-biotin-peroxidase detection system. Eye muscle tissues were obtained at surgery from 38 patients with TAO and 8 control subjects. HLA-DR expression on eye muscle cells was demonstrated in orbital tissue from 2 of 3 untreated patients and 2 of 35 patients who had been treated with orbital irradiation or corticosteroids, in all of whom lymphocytic infiltration was also demonstrated. HLA-DR was not detected on eye muscle cells fi om 8 normal controls studied. HLA-DR was expressed on endothelial cells and interstitial cells from almost all patients with TAO and all 8 control subjects. HSP-70 was detected in eye muscle cells from 31 of the patients with TAO, including all 3 untreated patients, and 3 of the controls. Although the degree of HSP-70 expression did not correlate with the severity of the ophthalmopathy, significant expression of HSP-70 in eye muscle cells was more often demonstrated in patients with eye disease of short duration (83%) than in those with disease of longer duration (33%). These results support the notion that eye muscle fiber is an important target of the orbital autoimmune reactions that characterize TAO.