Circadian expression and specific localization of synaptotagmin17 in the suprachiasmatic nucleus, the master circadian oscillator in mammals
Circadian expression and specific localization of synaptotagmin17 in the suprachiasmatic nucleus, the master circadian oscillator in mammals
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DOI：
10.1016/j.brainres.2022.148129
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发表时间：
2022-11-09
期刊：
影响因子：
2.9
通讯作者：
Shigeyoshi, Yasufumi
中科院分区：
文献类型：
--
作者：
Fujioka, Atsuko;Nagano, Mamoru;Shigeyoshi, Yasufumi
研究了突触结合蛋白（synaptotagmin，syt）17在大脑视交叉上核（SCN）的定位和功能。含Syt 17 mRNA的神经元主要分布在壳区，而在SCN的核和壳以及室旁下区（SPZ）也可检测到Syt 17免疫反应阳性的胞体和神经纤维。此外，电子显微镜分析显示SYT 17存在于粗面内质网（rER）、高尔基体（G）和神经元的大小泡中。SCN中Syt 17 mRNA表达呈昼夜节律，夜间光照抑制其表达。此外，自发活动节律的自由运行时间缩短Syt 17-缺失突变小鼠。这些发现表明SYT 17参与昼夜节律的调节。
The localization and function of synaptotagmin (syt)17 in the suprachiasmatic nucleus (SCN) of the brain, which is the master circadian oscillator, were investigated. The Syt17 mRNA-containing neurons were mainly situated in the shell region while SYT17 immunoreactive cell bodies and neural fibers were detected in the core and shell of the SCN and the subparaventricular zone (SPZ). Further, electron microscopy analysis revealed SYT17 in the rough endoplasmic reticulum (rER), Golgi apparatus (G), and large and small vesicles of neurons. Syt17 mRNA expression in the SCN showed a circadian rhythm, and light exposure at night suppressed its expression. In addition, the free running period of locomotor activity rhythm was shortened in Syt17-deletion mutant mice. These findings suggest that SYT17 is involved in the regulation of circadian rhythms.