D Rhamnose β-hederin inhibits migration and invasion of human breast cancer cell line MDA-MB-231
D Rhamnose β-hederin inhibits migration and invasion of human breast cancer cell line MDA-MB-231
复制标题
D 鼠李糖β-常春藤素抑制人乳腺癌细胞系MDA-MB-231的迁移和侵袭
DOI：
10.1016/j.bbrc.2017.11.081
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发表时间：
2018-01-01
影响因子：
3.1
通讯作者：
Ding, Qiang
中科院分区：
文献类型：
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作者：
Cheng, Lin;Xia, Tian-Song;Ding, Qiang
许多天然产物已被证明对包括乳腺癌在内的各种癌症的转移过程有抑制作用。从铁线莲中提取的鼠李糖β -hederin （DR β -h）是一种活性三萜皂苷D，可诱导乳腺癌细胞凋亡，但DR β -h对乳腺癌细胞转移的影响尚未明确。在这项研究中，我们证明了无细胞毒性浓度的DR β - h显著抑制伤口愈合迁移、通过腔室迁移和通过基质侵入。DR β - h还能调控MDA-MB-231细胞RNPC1、E-cadherin蛋白的表达。此外，RNPC1敲低降低了DR β - h诱导的MDA-MB-231细胞中RNPC1和E-Cadherin的上调。RNPC1的敲低降低了DR β - h的抗转移活性，这意味着DR β - h上调RNPC1的抗转移活性是其抗转移活性所必需的。这些结果表明DR β - h可能是治疗乳腺癌转移的潜在候选药物。(C) 2017爱思唯尔公司版权所有。
Many natural products have been shown to have inhibitory effects on the metastatic process of various cancers including breast cancer. An active triterpenoid saponin D Rhamnose beta-hederin (DR beta-H) from Clematis ganpiniana, has been known induce the apoptosis of breast cancer cells, but the effect of DR beta-H on the metastasis of breast cancer cells is largely unknown. In this study, we demonstrated that a non-cytotoxic concentration of DR beta-H markedly suppressed wound healing migration, migration through the chamber and invasion through the matrigel. In addition, DR beta-H regulated expression of RNPC1, E-cadherin proteins of MDA-MB-231 cells. Furthermore, RNPC1 knockdown decreased the DR beta-H-induced up regulation of RNPC1 and E-Cadherin in MDA-MB-231 cells. RNPC1 knockdown reduced the anti metastasis activities of DR beta-H, meaning that the up-rugulation of RNPC1 by DR beta-H is essential for its anti-metastatis activities. These results suggest that DR beta-H might be a potential therapeutic candidate for the treatment of breast cancer metastasis. (C) 2017 Elsevier Inc. All rights reserved.