Inositol 1,4,5-trisphosphate and ryanodine receptor distributions and patterns of acetylcholine- and caffeine-induced calcium release in cultured mouse hippocampal neurons
Inositol 1,4,5-trisphosphate and ryanodine receptor distributions and patterns of acetylcholine- and caffeine-induced calcium release in cultured mouse hippocampal neurons
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培养的小鼠海马神经元中肌醇1,4,5-三磷酸和兰尼碱受体的分布以及乙酰胆碱和咖啡因诱导的钙释放模式
DOI：
10.1523/jneurosci.15-04-02592.1995
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
M. Barish
中科院分区：
文献类型：
--
作者：
KJ Seymour;M. Barish
在培养的海马神经元上，比较了1，4，5-三磷酸肌醇受体（InsP 3R）和兰尼碱受体（RyR）的分布及其激活引起的细胞内钙离子浓度（[Ca ~（2+）]i）升高的模式。InsP 3R和RyR使用特异性抗体标记，并在星形神经元的胞体和树突中形成小聚集体。两种受体均分布于胞体。在树突的InsP 3R和RyR分布不均匀，InsP 3R被发现在所有地区，而RyR是最不密集的精细过程。使用Ca（2+）敏感的荧光染料和共聚焦显微镜在树突中测量乙酰胆碱（通过毒蕈碱受体激活InsP 3受体）和咖啡因（刺激ryanodine受体）引起的[Ca 2 +]i增加。Ca 2+对乙酰胆碱的反应是短暂的，在近端和远端树突状区域观察到。与此相反，咖啡因诱导的反应持续和限制近端树突。因此，细树突中的钙释放模式反映了InsP 3R和RyR的分布。在没有外部钙的情况下观察到对乙酰胆碱和咖啡因的钙反应，因此依赖于Ca 2+的释放。Ca 2+反应表现出局部波动和响应延迟时间的变化。胞体中InsP 3R和RyR的顺序激活导致了Ca 2+释放的相互阻断。InsP 3门控和Ca（2+）诱导的Ca（2+）释放在空间上是不同的，但相互作用，机制的存在可能是重要的振荡和传播的Ca（2+）波的海马神经元胞体和树突。
The distributions of inositol 1,4,5-trisphosphate and ryanodine receptors (InsP3R and RyR) and the patterns of increase in intracellular calcium ion concentration ([Ca2+]i) elicited by their activation were compared in cultured hippocampal neurons. InsP3R and RyR were labeled using specific antibodies and formed small aggregations in the somata and dendrites of pyramidally shaped neurons. Both receptors were densest in somata. In dendrites the InsP3R and RyR were not distributed homogeneously; InsP3R was found in all regions, while RyR was least dense in fine processes. Increases in [Ca2+]i elicited by acetylcholine (to activate InsP3 receptors via muscarinic receptors) and caffeine (to stimulate ryanodine receptors) were measured in dendrites using Ca(2+)-sensitive fluorescent dyes and confocal microscopy. Ca2+ responses to acetylcholine were transient and observed in proximal and distal dendritic regions. In contrast, caffeine-induced responses were sustained and restricted to proximal dendrites. Thus the patterns of calcium release in fine dendrites mirrored the distributions of InsP3R and RyR. Calcium responses to both acetylcholine and caffeine were observed in the absence of external calcium and thus were dependent on Ca2+ release. Ca2+ responses showed localized fluctuations and variations in response delay times. Sequential activation of InsP3R and RyR in somata resulted in mutual occlusion of Ca2+ release. The existence of InsP3-gated and Ca(2+)- induced Ca2+ release as spatially distinct, but mutually interacting, mechanisms may be important in the generation of oscillations and propagating Ca2+ waves in somata and dendrites of hippocampal neurons.
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DOI：
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