Impedance spectroscopy flow cytometry: On-chip label-free cell differentiation
Impedance spectroscopy flow cytometry: On-chip label-free cell differentiation
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DOI：
10.1002/cyto.a.20141
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发表时间：
2005-06-01
期刊：
影响因子：
3.7
通讯作者：
Renaud, P
中科院分区：
文献类型：
--
作者：
Cheung, K;Gawad, S;Renaud, P
背景资料：在这项研究中使用的微加工阻抗谱流式细胞仪允许快速介电特性的细胞群体与一个简单的微流体通道。在宽频率范围内的阻抗测量提供关于细胞大小、膜电容和细胞质电导率作为频率的函数的信息。振幅，不透明度和相位信息可以用于区分不同的细胞群体之间，而不使用cellmarkers.Methods：聚苯乙烯珠，红细胞（RBC），鬼，和固定在戊二醛中的RBC通过一个微型流式细胞仪和单独测量通过使用两个同时应用的离散频率。在350 kHz至20 MHz.Results的频率范围内，以每分钟1，000个细胞的速度对其进行表征：细胞大小易于以亚微米精度测量。使用不透明度区分聚苯乙烯珠和RBC。使用相位信息区分RBC和血影，而使用不透明度区分RBC和固定RBC。使用浓度增加的戊二醛固定的RBC显示出增加的不透明度。这种增加的不透明度与细胞质电导率降低和膜电容降低，这两个蛋白质cross-linking.Conclusions的结果：这项工作提出了无标记的细胞分化的芯片上流式细胞仪的基础上，阻抗谱，这将是一个强大的工具，细胞特性。(c)2005 Wiley-Liss，Inc.
Background: The microfabricated impedance spectroscopy flow cytometer used in this study permits rapid dielectric characterization of a cell population with a simple microfluidic channel. Impedance measurements over a wide frequency range provide information on cell size, membrane capacitance, and cytoplasm conductivity as a function of frequency. The amplitude, opacity, and phase information can be used for discrimination between different cell populations without the use of cell markers.Methods: Polystyrene beads, red blood cells (RBCs), ghosts, and RBCs fixed in glutaraldehyde were passed through a microfabricated flow cytometer and measured individually by using two simultaneously applied discrete frequencies. The cells were characterized at 1,000 per minute in the frequency range of 350 kHz to 20 MHz.Results: Cell size was easily measured with submicron accuracy. Polystyrene beads and RBCs were differentiated using opacity. RBCs and ghosts were differentiated using phase information, whereas RBCs and fixed RBCs were differentiated using opacity RBCs fixed using increasing concentrations of glutaraldehyde showed increasing opacity. This increased opacity was linked to decreased cytoplasm conductivity and decreased membrane capacitance, both resulting from protein cross-linking.Conclusions: This work presents label-free differentiation of cells in an on-chip flow cytometer based on impedance spectroscopy, which will be a powerful tool for cell characterization. (c) 2005 Wiley-Liss, Inc.