Antinociceptive and anti-inflammatory effects of Brazilian red propolis extract and formononetin in rodents
Antinociceptive and anti-inflammatory effects of Brazilian red propolis extract and formononetin in rodents
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DOI：
10.1016/j.jep.2015.07.022
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发表时间：
2015-09-15
影响因子：
5.4
通讯作者：
Gomes, Margarete Zanardo
中科院分区：
文献类型：
--
作者：
Cavendish, Rodrigo Lima;Santos, Jandson de Souza;Gomes, Margarete Zanardo
民族药物学相关性：蜂胶因其广泛的生物活性而被用作世界各地的民间药物已有数百年的历史。红蜂胶是该材料的巴西新品种，其化学成分异常，包括芒柄花素和生物查宁A等异黄酮类成分。由于绿色和红色品种的蜂胶传统上都被用作药物，并且没有标签区分，因此研究红蜂胶提取物的活性对于澄清该产品是否具有与商业蜂胶相同的活性具有重要意义。在这项工作中，我们在实验模型上证明了蜂胶水乙醇提取物(HERP)及其生物标志物芒柄花素作为抗伤害和抗炎药物的潜在作用。材料和方法：HERP的化学特征通过高效液相色谱/DAD分析。采用小鼠抗伤害性(醋酸、福尔马林和谷氨酸注射)和抗炎(角叉菜胶致小鼠后足肿胀和腹膜炎)模型，评价HERP(3、10和30 mg/kg)和芒柄花素(10 mg/kg)的生物活性。还进行了开阔场地试验。结果：在HERP中鉴定出蜂胶的主要生物标志物之一--芒柄花素，含量为21.62 mg/g。HERP(10 mg/kg，30 mg/kg)和芒柄花素(10 mg/kg，P
Ethnopharmacological relevance: Propolis has been used as a folk medicine for centuries around the world due to its wide spectrum of biological activities. The red propolis, a new Brazilian variety of this apimaterial, has presented an unusual chemical composition, including isoflavones such as formononetin and biochanin A. Since both the green and red varieties of propolis are traditionally used as medicine and commercialized with no label differentiation, the study of the activities of red propolis extract has become important in order to clarify whether this product has the same activities as commercial ones. In this work, we demonstrated the potential action of the hydroalcoholic extract of red propolis (HERP) and its biomarker, formononetin, as antinociceptive and anti-inflammatory drugs on experimental models.Materials and methods: The HERP was chemically characterised by HPLC/DAD analyses. The biological activities of the HERP (3, 10, and 30 mg/kg) and formononetin (10 mg/kg) were evaluated using the antinociceptive (acetic acid, formalin, and glutamate injections) and anti-inflammatory (carrageenan-induced hindpaw oedema and peritonitis) models in mice after oral administration. The open field test was also performed. Results: Formononetin, one of the main biomarker of red propolis, was identified in the HERP (21.62 mg/g). Pretreatment with the HERP (10 and 30 mg/kg) and formononetin (10 mg/kg) produced reduction (P