MURINE INTESTINAL MUCINS INHIBIT ROTAVIRUS INFECTION
MURINE INTESTINAL MUCINS INHIBIT ROTAVIRUS INFECTION
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DOI：
10.1016/0016-5085(93)90013-3
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发表时间：
1993-07-01
期刊：
影响因子：
29.4
通讯作者：
BASS, DM
中科院分区：
文献类型：
--
作者：
CHEN, CC;BAYLOR, M;BASS, DM
背景：黏液蛋白是一种聚合糖蛋白，是胃肠道黏液层的主要组成部分。这些研究旨在确定小鼠肠粘蛋白是否抑制轮状病毒感染。方法：刮取小鼠肠段，经CsCl梯度离心和蔗糖4B层析纯化获得小鼠肠粘蛋白。用黏液-轮状病毒混合物感染后，通过免疫过氧化物酶染色细胞的定量测定来确定感染的抑制作用。结果：从哺乳小鼠和成年小鼠中提取的粗制和纯化的肠粘蛋白对猴轮状病毒、恒河轮状病毒（RRV）的复制具有强抑制作用，但对其他轮状病毒的复制具有弱抑制作用。在所有的制剂中，结肠粘蛋白是比小肠粘蛋白更有效的RRV抑制剂。乳汁黏液比成人黏液更能有效地中和RRV。在一组轮状病毒重组中，对粘蛋白抑制的易感性与人O型红细胞血凝能力和RRV基因4相关。小鼠肠粘蛋白抑制RRV与MA104细胞的结合，提示抑制病毒与细胞的附着可能是RRV中和的机制。巯基乙醇或神经氨酸酶抑制粘蛋白的抗rrv活性，表明粘蛋白的聚合结构和唾液酸含量具有重要的功能。结论：这些发现提示肠粘蛋白是某些轮状病毒感染的屏障。
Background:Mucin, a population of polymeric glycoproteins, constitutes the primary component of the mucus layer that overlies the gastrointestinal tract. These studies aimed to determine whether murine intestinal mucins inhibit rotavirus infection.Methods:Murine intestinal mucins were obtained by scraping segments of mouse intestine and purification via CsCl gradient centrifugation and sepharose 4B chromatography. Inhibition of infection was determined by quantitation of immunoperoxidase-stained cells after infection with mucin-rotavirus mixtures.Results:Crude and purified intestinal mucins from suckling and adult mice are potent inhibitors of replication of a simian rotavirus, rhesus rotavirus (RRV), but weak inhibitors of other rotaviruses. In all preparations, colonic mucins were more potent inhibitors of RRV than small intestinal mucins. Suckling mucins neutralized RRV more effectively than adult mucins. In a panel of rotavirus reassortants, susceptibility to mucin inhibition correlated with the ability to hemagglutinate human type O erythrocytes and with RRV gene 4. Murine intestinal mucin inhibited RRV binding to MA104 cells, suggesting inhibition of virus-cell attachment to be the mechanism for neutralization. Mercaptoethanol or neuraminidase inhibited mucins' anti-RRV activities, implying the functional importance of mucins' polymeric structure and sialic acid content.Conclusions:These findings suggest that intestinal mucins represent a barrier to certain rotavirus infections.