Comprehensive esophageal microscopy by using optical frequency-domain imaging (with video)
Comprehensive esophageal microscopy by using optical frequency-domain imaging (with video)
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DOI：
10.1016/j.gie.2006.08.009
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发表时间：
2007-05-01
影响因子：
7.7
通讯作者：
Bouma, Brett E.
中科院分区：
文献类型：
--
作者：
Vakoc, Benjamin J.;Shishko, Milen;Bouma, Brett E.
背景：光学相干断层扫描（OCT）已被用于高分辨率内镜成像和诊断特征性肠化生、不典型增生和食管粘膜内癌。然而，目前OCT系统相对较慢的图像采集速率抑制了宽视场成像，限制了OCT在Barrett食管患者诊断成像中的临床应用。目的：本研究描述了一种新的光学成像技术——光学频域成像（OFDI），它起源于OCT，能够以显微分辨率对大食道段进行全面成像。设计：开发了用于内窥镜成像的OFDI系统原型。该系统与球囊中心导管结合使用，对猪食管远端进行全面成像。结果：获得了4.5 cm长段的粘膜和部分固有肌层的体积图像。图像分辨率为7 μ m深，平行于管腔30 μ m，清晰描绘了各粘膜层。三维数据集用于创建横截面显微图像，以及食管的血管图。用多普勒流处理观察粘膜下血管和毛细血管。结论：OFDI在体内对食管远端进行全面显微成像是可行的。该技术在获得大粘膜区域组织微观结构的详细信息方面的独特能力可能为改善Barrett食管患者的管理开辟新的可能性。
Background: Optical coherence tomography (OCT) has been used for high-resolution endoscopic imaging and diagnosis of specialized intestinal metaplasia, dysplasia, and intramucosal carcinoma of the esophagus. However, the relatively slow image-acquisition rate of the present OCT systems inhibits wide-field imaging and limits the clinical utility of OCT for diagnostic imaging in patients with Barrett's esophagus.Objective: This study describes a new optical imaging technology optical frequency-domain imaging (OFDI), derived from OCT, that enables comprehensive imaging of large esophageal segments with microscopic resolution.Design: A prototype OFDI system was developed for endoscopic imaging. The system was used in combination with a balloon-centering catheter to comprehensively image the distal esophagus in swine.Results: Volumetric images of the mucosa and portions of the muscularis propria were obtained for 4.5-cm-long segments. Image resolution was 7 mu m in depth and 30 mu m parallel to the lumen, and provided clear delineation of each mucosal layer. The 3-dimensional data sets were used to create cross-sectional microscopic images, as well as vascular maps of the esophagus. Submucosal vessels and capillaries were visualized by using Doppler-flow processing.Conclusions: Comprehensive microscopic imaging of the distal esophagus in vivo by using OFDI is feasible. The unique capabilities of this technology for obtaining detailed information of tissue microstructure over large mucosal areas may open up new possibilities for improving the management of patients with Barrett's esophagus.