The molecular portrait of in vitro growth by meta-analysis of gene-expression profiles -: art. no. r65
The molecular portrait of in vitro growth by meta-analysis of gene-expression profiles -: art. no. r65
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DOI：
10.1186/gb-2005-6-8-r65
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发表时间：
2005-01-01
期刊：
影响因子：
12.3
通讯作者：
Ernberg, I
中科院分区：
文献类型：
--
作者：
Sandberg, R;Ernberg, I
背景资料：细胞系作为肿瘤和组织的模型系统在分子生物学中是必不可少的，尽管它们仅近似于组织中的体内细胞的性质。在体外条件下，细胞系已被选择了很长一段时间，影响许多特定的细胞通路和processes.Results：要确定长期在体外选择引起的转录变化，我们进行了基因表达荟萃分析，并比较了60肿瘤细胞系（9个组织来源），135人组织和176肿瘤组织样本。使用微阵列的显著性分析，我们证明了与组织相比，细胞系显示出约7，000个研究基因中约30%的统计学显著差异表达。大多数差异与体外较高的增殖率和破坏的组织结构有关。因此，参与细胞周期进程，大分子加工和营业额，和能量代谢的基因在细胞系上调，而细胞粘附分子和膜信号蛋白下调。结论：详细的分子理解细胞如何适应在体外环境是重要的，因为它既会增加我们的理解组织的组织结构，并导致在一个精致的分子画像的增殖。它将进一步指出何时使用永生化细胞系，或何时需要使用三维培养、原代细胞培养或组织活检。
Background: Cell lines as model systems of tumors and tissues are essential in molecular biology, although they only approximate the properties of in vivo cells in tissues. Cell lines have been selected under in vitro conditions for a long period of time, affecting many specific cellular pathways and processes.Results: To identify the transcriptional changes caused by long term in vitro selection, we performed a gene-expression meta-analysis and compared 60 tumor cell lines ( of nine tissue origins) to 135 human tissue and 176 tumor tissue samples. Using significance analysis of microarrays we demonstrated that cell lines showed statistically significant differential expression of approximately 30% of the approximately 7,000 genes investigated compared to the tissues. Most of the differences were associated with the higher proliferation rate and the disrupted tissue organization in vitro. Thus, genes involved in cell-cycle progression, macromolecule processing and turnover, and energy metabolism were upregulated in cell lines, whereas cell adhesion molecules and membrane signaling proteins were downregulated.Conclusion: Detailed molecular understanding of how cells adapt to the in vitro environment is important, as it will both increase our understanding of tissue organization and result in a refined molecular portrait of proliferation. It will further indicate when to use immortalized cell lines, or when it is necessary to instead use three-dimensional cultures, primary cell cultures or tissue biopsies.