Tracer and electrical coupling of rat suprachiasmatic nucleus neurons
Tracer and electrical coupling of rat suprachiasmatic nucleus neurons
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DOI：
10.1016/s0306-4522(96)00539-8
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发表时间：
1997-04-01
期刊：
影响因子：
3.3
通讯作者：
Allen, CN
中科院分区：
文献类型：
--
作者：
Jiang, ZG;Yang, YQ;Allen, CN
使用含有示踪神经生物素的电极对视交叉上核的单个神经元进行全细胞记录，导致 30% 的病例中多个神经元被染色。通常，一个神经元被树突状过程深染，轴突清晰可见，而其他神经元则被浅染。深染色的细胞被识别为记录的神经元，并与一到五个浅染色的神经元偶联示踪剂。记录的具有示踪剂耦合细胞的神经元的静息膜电位、输入膜电导、膜电容、衰减时间常数和最大 H 电流幅度与未显示示踪剂耦合的神经元没有显着差异。当突触传递被无钙/Mn2+溶液阻断时，刺激视前区会激活一些神经元的逆向动作电位或全部或全部缓慢的内向电流。小而缓慢的内向电流并未与顺向激活的动作尖峰“碰撞”，这表明该电流代表来自电紧张耦合神经元的信号。此外，来自邻近细胞放电的双相场电流的频率因记录细胞的去极化而增加，并因记录细胞的超极化而减少。这些数据证明了视交叉上核神经元的化学和电低阻耦合，这对于合成视交叉上核昼夜节律可能很重要。 (C) 1997 国际广播组织。由爱思唯尔科学有限公司出版
Whole-cell recording from single neurons of the suprachiasmatic nucleus with an electrode containing the tracer neurobiotin resulted in the staining of multiple neurons in 30% of the cases. Typically, one neuron was darkly stained with dendritic processes and an axon clearly visible while other neurons were lightly stained. The darkly-stained cells were identified as the recorded neuron and tracer-coupled to one to five lightly stained neurons. The resting membrane potential, input membrane conductance, membrane capacitance, the decay time constant and the maximum H-current amplitude of the recorded neurons with tracer-coupled cells were not significantly different from those of neurons not showing tracer coupling. Stimulation of the preoptic area activated an antidromic action potential or an all-or-none small slow inward current in some neurons when the synaptic transmission was blocked by a calcium-free/Mn2+ solution. The small slow inward current did not ''collide'' with an orthodromically activated action spike suggesting that the current represents the signal from an electrotonically-coupled neuron. In addition, the frequency of biphasic field currents from a neighbouring cell firing were increased by depolarization and decreased by hyperpolarization of the recorded cell.These data demonstrate a chemical and electrical low-resistance coupling of suprachiasmatic nucleus neurons, which could be important in synthesizing the suprachiasmatic nucleus circadian rhythm. (C) 1997 IBRO. Published by Elsevier Science Ltd.