Zebrafish collagen XII is present in embryonic connective tissue sheaths (fascia) and basement membranes
Zebrafish collagen XII is present in embryonic connective tissue sheaths (fascia) and basement membranes
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DOI：
10.1016/j.matbio.2008.09.580
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发表时间：
2009-01-01
期刊：
影响因子：
6.9
通讯作者：
Ruggiero, Florence
中科院分区：
文献类型：
--
作者：
Bader, Hannah L.;Keene, Douglas R.;Ruggiero, Florence
结缔组织确保身体组织的凝聚力，但也形成专门的结构，如肌腱和骨骼。胶原XII可以通过桥接胶原纤维来增强结缔组织的稳定性，但其功能尚不清楚。在这里，我们使用斑马鱼模型来可视化其在整个生物体中的表达模式。斑马鱼col 12 a1基因与四足动物col 12 a1基因的小亚型同源。与报道的小同种型的生物化学数据一致，斑马鱼胶原蛋白XII α 1链被表征为在~ 200 kDa处迁移的胶原酶敏感带。使用新产生的多克隆抗体和反义探针，我们进行了全面的分析，其在发育中的斑马鱼的表达。胶原蛋白XII表现出比以前认为的更广泛的表达模式：它在包裹身体组织和器官的结缔组织鞘（筋膜）中普遍表达。如巩膜、脑膜、眼表、水平肌隔和垂直肌隔等。胶原蛋白XII也被检测到头部间充质，咽弓和脊髓内，它首先在内，然后在底板的外侧边缘和背中线。此外，双重免疫荧光染色与层粘连蛋白和免疫金电子显微镜显示，胶原XII与基底膜。这些数据表明，胶原XII通过稳定筋膜和连接筋膜与基底膜参与组织凝聚。(C)2008 Elsevier B. V.保留所有权利。
Connective tissues ensure the cohesion of the tissues of the body, but also form specialized structures such as tendon and bone. Collagen XII may enhance the stability of connective tissues by bridging collagen fibrils, but its function is still unclear. Here, we used the zebrafish model to visualize its expression pattern in the whole organism. The zebrafish col12a1 gene is homologous to the small isoform of the tetrapod col12a1 gene. In agreement with the biochemical data reported for the small isoform, the zebrafish collagen XII alpha 1 chain was characterized as a collagenase sensitive band migrating at -200 kDa. Using newly generated polyclonal antibodies and anti-sense probes, we performed a comprehensive analysis of its expression in developing zebrafish. Collagen XII exhibited a much broader expression pattern than previously thought: it was ubiquitously expressed in the connective tissue sheaths (fascia) that encase the tissues and organs of the body. For example, it was found in sclera, meninges, epimysia and horizontal and vertical myosepta. Collagen XII was also detected in head mesenchyme, pharyngeal arches and within the spinal cord, where it was first expressed within and then at the lateral borders of the floor plate and at the dorsal midline. Furthermore, double immunofluorescence staining with laminin and immunogold electron microscopy revealed that collagen XII is associated with basement membranes. These data suggest that collagen XII is implicated in tissue cohesion by stabilizing fascia and by linking fascia to basement membranes. (C) 2008 Elsevier B.V. All rights reserved.