Development of Confocal Laser Scanning Microscope/Atomic Force Microscope System for Force Curve Measurement
Development of Confocal Laser Scanning Microscope/Atomic Force Microscope System for Force Curve Measurement
复制标题
用于力曲线测量的共焦激光扫描显微镜/原子力显微镜系统的开发
DOI：
10.1143/jjap.43.4580
复制
发表时间：
2004
影响因子：
1.5
通讯作者：
A. Ikai
中科院分区：
文献类型：
--
作者：
T. Kodama;H. Ohtani;H. Arakawa;A. Ikai
将倒置激光扫描显微镜（CLSM）与原子力显微镜（AFM）相结合，建立了一套共焦激光扫描显微镜（CLSM）/原子力显微镜（AFM）系统，用于液体中的光谱测量和力曲线测量。我们观察到共振能量转移从供体染料连接到AFM探针受体染料固定在基板表面上。我们同时进行了接触面积的光谱测量和力曲线测量，并证明了这些实验结果的实验技术和它的有效性。
We developed a confocal laser scanning microscope (CLSM) / atomic force microscope (AFM) system by combining an inverted CLSM with an AFM for simultaneous spectroscopic measurement and force curve measurement in liquid. We observed resonance energy transfer from donor dyes attached to an AFM probe to acceptor dyes immobilized on the substrate surface. We simultaneously performed the spectroscopic measurement of contact area and force curve measurement and demonstrated the experimental technique and its validity with these experimental results.