On (GGLGY) synthetic repeating sequences of lamprin and analogous sequences
On (GGLGY) synthetic repeating sequences of lamprin and analogous sequences
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DOI：
10.1016/s0945-053x(01)00139-1
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发表时间：
2001-07-01
期刊：
影响因子：
6.9
通讯作者：
Tamburro, AM
中科院分区：
文献类型：
--
作者：
Bochicchio, B;Pepe, A;Tamburro, AM
Keeley 及其同事在七鳃鳗环状软骨最重要的基质蛋白七鳃鳗蛋白中发现了重复序列 GGLGY。类似的序列也出现在其他蛋白质中，即弹性蛋白、蜘蛛丝蛋白、蜘蛛小壶腹丝蛋白、昆虫等的绒毛膜或蛋壳膜的基质蛋白中。我们合成了 (GGLGY)(n)，n = 1, 2, 6，因为该序列在聚集的蛋白质中重复了六次。对溶液和固态的肽进行了研究。由于圆二色光谱主要由芳香族贡献，我们合成了 GGLGF 和 GGLGA 以仔细解释圆二色光谱。构象分析表明所有合成肽确实采用相同的二级结构。在溶液中，肽呈现出具有大量 PPII 结构的灵活构象。在固态 PPII 中，β 折叠片和 β 转角可能共存。 (C) 2001 Elsevier Science B.V./国际基质生物学学会。版权所有。
The repetitive sequence GGLGY was found in lamprin, the most important matrix protein of lamprey annular cartilage by Keeley and co-workers. Similar sequences appear also in other proteins, i.e. elastin, spidroin, spider minor ampullate silk proteins, in matrix proteins of the chorion or egg shell membrane of insects and others. We synthesized (GGLGY)(n), n = 1, 2, 6, because the sequence is repeated six times in the aggregated protein. The peptides were studied both in solution and in the solid state. Because the CD spectra were dominated by aromatic contribution, we synthesized GGLGF and GGLGA in order to carefully interpret the CD spectra. The conformational analysis suggests that all synthetic peptides do adopt the same secondary structure. In solution the peptides present a flexible conformation with a significant amount of PPII structure. In the solid state PPII, beta -pleated-sheets and beta -turns possibly co-exist. (C) 2001 Elsevier Science B.V./International Society of Matrix Biology. All rights reserved.