Fecal indicator bacteria, direct pathogen detection, and microbial community analysis provide different microbiological water quality assessment of a tropical urban marine estuary
Fecal indicator bacteria, direct pathogen detection, and microbial community analysis provide different microbiological water quality assessment of a tropical urban marine estuary
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粪便指示菌、直接病原体检测和微生物群落分析为热带城市海洋河口提供不同的微生物水质评估
DOI：
10.1016/j.watres.2020.116280
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发表时间：
2020
期刊：
影响因子：
12.8
通讯作者：
Yan, Tao
中科院分区：
文献类型：
--
作者：
Saingam, Prakit;Li, Bo;Yan, Tao
城市海洋河口经常受到微生物污染的影响，这损害了使用并严重影响人类健康，而在没有污水泄漏报告的情况下，对城市海洋河口的微生物水质知之甚少。本研究使用的热带城市海洋河口，阿拉阿威运河在檀香山，夏威夷，作为模型系统，比较粪便指示细菌（FIB）的浓度，细菌病原体的配置文件，和微生物群落结构。结果显示，FIB大肠杆菌的几何平均数为132 CFU/100 mL（n=28），高于肠球菌（18 CFU/100 mL）和产气荚膜梭菌（21 CFU/100 mL）。在沙门氏菌、弯曲杆菌、单核细胞增生李斯特菌和副溶血性弧菌这4种培养方法所针对的病原体中，只有V.检出副溶血性，检出频率高。通过16 S rRNA基因扩增子测序的微生物群落分析也表明水中弧菌的高流行率。病原菌检测模式和微生物群落结构与FIB浓度分布无显著相关性。总之，这些结果突出了使用传统FIB评估热带城市海洋河口环境中水微生物质量的局限性，表明需要更全面的微生物风险评估方法，如直接检测病原体。
Urban marine estuaries are often impacted by microbiological contamination that impairs use and affects human health acutely, while limited is known about microbiological water quality in urban marine estuaries in the absence of reported sewage spills. This study used a tropical urban marine estuary, the Ala Wai Canal in Honolulu, Hawaii, as the model system to compare fecal indicator bacteria (FIB) concentrations, bacterial pathogen profiles, and microbial community structures. The FIBEscherichia coliexhibited higher geometric mean 132 CFU/100mL (n=28) than those of enterococci (18 CFU/100mL) andClostridium perfringens(21 CFU/100mL). Amongst the four pathogens targeted by cultivation methods (Salmonella, Campylobacter, Listeria monocytogenesandVibrio parahaemolyticus), only wasV. parahaemolyticusdetected and was detected at high frequency. Microbial community analysis through 16S rRNA gene amplicon sequencing also indicated the high prevalence ofVibrioin the water. The pathogen detection patterns and microbial community structure showed no significant correlation with FIB concentration profiles. Together, the results highlight the limitation of using traditional FIB in assessing water microbiological quality in the tropical urban marine estuary environment, indicating the need for more comprehensive microbial risk assessment approaches such as direct detection of pathogens.