Two potato proteins, including a novel RING finger protein (HIP1), interact with the potyviral multifunctional protein HCpro
Two potato proteins, including a novel RING finger protein (HIP1), interact with the potyviral multifunctional protein HCpro
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DOI：
10.1094/mpmi.2003.16.5.405
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发表时间：
2003-05-01
影响因子：
3.5
通讯作者：
Valkonen, JPT
中科院分区：
文献类型：
--
作者：
Guo, DY;Spetz, C;Valkonen, JPT
马铃薯病毒辅助成分蛋白酶（HCpro）是一种多功能蛋白，通过与假定的宿主蛋白相互作用发挥其细胞功能。在这项研究中，使用酵母双杂交系统在马铃薯叶 cDNA 文库中筛选了马铃薯病毒 A (PVA)（马铃薯病毒属）编码的 HCpro 的细胞蛋白伴侣。获得了两种细胞蛋白，它们与酵母中的 PVA HCpro 特异性相互作用，并且在所使用的两种体外结合测定中。这两种蛋白质均由马铃薯基因组中的单拷贝基因编码。对推导的氨基酸序列的分析表明，两个 HCpro 相互作用蛋白中的一个 (HIP1) 是一种新型 RING 指蛋白。另一种蛋白质 (HIP2) 的序列与数据库中提供的蛋白质序列没有相似之处，并且具有已知的生物学功能。
Potyviral helper-component proteinase (HCpro) is a multifunctional protein exerting its cellular functions in interaction with putative host proteins. In this study, cellular protein partners of the HCpro encoded by Potato virus A (PVA) (genus Potyvirus) were screened in a potato leaf cDNA library using a yeast two-hybrid system. Two cellular proteins were obtained that interact specifically with PVA HCpro in yeast and in the two in vitro binding assays used. Both proteins are encoded by single-copy genes in the potato genome. Analysis of the deduced amino acid sequences revealed that one (HIP1) of the two HCpro interactors is a novel RING finger protein. The sequence of the other protein (HIP2) showed no resemblance to the protein sequences available from databanks and has known biological functions.