Potential Role of Long Non-Coding RNA in Osteogenic Differentiation of Human Periodontal Ligament Stem Cells
Potential Role of Long Non-Coding RNA in Osteogenic Differentiation of Human Periodontal Ligament Stem Cells
复制标题
长非编码RNA在人牙周膜干细胞成骨分化中的潜在作用
DOI：
10.1902/jop.2016.150592
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发表时间：
2016-07-01
影响因子：
4.3
通讯作者：
Ge, Yihong
中科院分区：
文献类型：
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作者：
Qu, Qian;Fang, Fuchun;Ge, Yihong
背景：长非编码RNA(Long Non-Coding RNAs，LncRNAs)正逐渐成为真核基因表达的重要调节因子，并被证明可以调节发育和分化的各种模块成分。然而，目前对lncRNAs在人牙周膜干细胞成骨分化调控中的作用还知之甚少。方法：应用基因芯片技术分析lncRNA和信使RNA在成骨分化过程中的表达模式。采用定量逆转录聚合酶链式反应对芯片数据进行验证。通过1)聚类分析、2)基因本体论(GO)和3)通径分析揭示了候选基因的生物学功能。构建了编码-非编码基因共表达网络，以研究lncRNAs和成骨相关mRNAs的潜在调控作用。结果：成骨诱导后，3,557个mRNAs和2,171个lncRNAs差异表达，其中994个lncRNAs上调，1,177个lncRNAs下调(折叠式变化；2.0或
Background: Long non-coding RNAs (lncRNAs) are emerging as important regulators of eukaryotic gene expression and have been shown to regulate various modular components of development and differentiation. However, the roles of lncRNAs in the regulation of osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) remain poorly understood.Methods: Expression patterns of lncRNA and messenger RNA (mRNA) during osteogenic differentiation were profiled using microarray analysis. Quantitative reverse transcription polymerase chain reaction was performed to validate the microarray data. Biologic functions of candidates were revealed by: 1) cluster analysis; 2) gene ontology (GO); and 3) pathway analysis. Coding-non-coding gene coexpression (CNC) networks were constructed to investigate potential regulatory roles of lncRNAs and osteogenesis-related mRNAs.Results: After osteoinduction, 3,557 mRNAs and 2,171 lncRNAs were differentially expressed, of which 994 lncRNAs were upregulated and 1,177 were downregulated (fold change >2.0 or