Stress-induced transcription of satellite III repeats.
Stress-induced transcription of satellite III repeats.
复制标题
应力诱导的卫星III重复转录。
DOI：
10.1083/jcb.200306104
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发表时间：
2004-01-05
影响因子：
7.8
通讯作者：
Vourc'h, Claire
中科院分区：
文献类型：
--
作者：
Jolly, Caroline;Metz, Alexandra;Govin, Jerome;Vigneron, Marc;Turner, Bryan M;Khochbin, Saadi;Vourc'h, Claire
哺乳动物细胞暴露于应激诱导热休克转录因子1（HSF 1）的激活和随后的热休克基因的转录。热休克反应的激活也与HSF 1在称为核应激颗粒的一些核结构内的快速重定位相关。这些应力诱导的结构，主要形成在9q12区域在人类通过直接绑定的HSF 1卫星III重复，不共定位与已知的热休克蛋白基因的转录位点。在本文中，我们表明，核应力颗粒对应于RNA聚合酶II转录工厂，卫星III重复转录成大而稳定的RNA，保持与9q12区域，甚至在整个有丝分裂。这项工作不仅揭示了在人类细胞中存在一种新的主要热诱导转录本，它可能在染色质结构中发挥作用，而且还提供了证据，证明在一个被认为是异染色质和沉默的基因座内存在转录活性。
Exposure of mammalian cells to stress induces the activation of heat shock transcription factor 1 (HSF1) and the subsequent transcription of heat shock genes. Activation of the heat shock response also correlates with a rapid relocalization of HSF1 within a few nuclear structures termed nuclear stress granules. These stress-induced structures, which form primarily on the 9q12 region in humans through direct binding of HSF1 to satellite III repeats, do not colocalize with transcription sites of known hsp genes. In this paper, we show that nuclear stress granules correspond to RNA polymerase II transcription factories where satellite III repeats are transcribed into large and stable RNAs that remain associated with the 9q12 region, even throughout mitosis. This work not only reveals the existence of a new major heat-induced transcript in human cells that may play a role in chromatin structure, but also provides evidence for a transcriptional activity within a locus considered so far as heterochromatic and silent.