Oral vaccination with an adenovirus-vectored vaccine protects against botulism
Oral vaccination with an adenovirus-vectored vaccine protects against botulism
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DOI：
10.1016/j.vaccine.2012.12.054
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发表时间：
2013-02-01
期刊：
影响因子：
5.5
通讯作者：
Zeng, Mingtao
中科院分区：
文献类型：
--
作者：
Chen, Shan;Xu, Qingfu;Zeng, Mingtao
We have previously shown that an adenovirus vectored vaccine delivered intramuscularly or intranasally was effective in protection against botulism in a mouse model. The adenoviral vector encodes a human codon-optimized heavy chain C-fragment (H(C)50) of botulinum neurotoxin type C (BoNT/C). Here, we evaluate the same vaccine candidate as an oral vaccine against BoNT/C in a mouse model. To elicit protective immunity, the mice were orally vaccinated with a single dose of 1 x 10(4) to 1 x 10(7) plaque forming units (pfu) of the adenoviral vector. The immune sera, collected six weeks after oral vaccination with 2 x 10(7) pfu adenovirus, have shown an ability to neutralize the biological activity of BoNT/C in vitro. Additionally, animals receiving a single dose of 2 x 10(6) pfu adenovirus or greater were completely protected against challenge with 100 x MLD50 of BoNT/C. The data demonstrated the feasibility to develop an adenovirus-based oral vaccine against botulism. (C) 2012 Elsevier Ltd. All rights reserved.
