CircRNA_103948 inhibits autophagy in colorectal cancer in a ceRNA manner (Retracted article. See vol. 1519, pg. 211, 2023)
CircRNA_103948 inhibits autophagy in colorectal cancer in a ceRNA manner (Retracted article. See vol. 1519, pg. 211, 2023)
复制标题
CircRNA_103948以ceRNA方式抑制结直肠癌自噬
DOI：
10.1111/nyas.14679
复制
发表时间：
2021-09-03
影响因子：
5.2
通讯作者：
Mu, Zepeng
中科院分区：
文献类型：
--
作者：
Zhang, Nanyang;Zhang, Xianxiang;Mu, Zepeng
环状RNA（circRNA）与许多类型的癌症有关;然而，circRNA在结直肠癌（CRC）中的表达和作用仍然知之甚少。在这项研究中，进行了circRNA微阵列检测以检测CRC中异常表达的circRNA，并使用组织阵列来确定CRC患者的预后。使用细胞计数试剂盒-8、克隆形成、伤口愈合和transwell试验评价体外细胞功能，并设计小鼠皮下肿瘤模型进行体内分析。共聚焦激光扫描和透射电子显微镜观察自噬。采用qPCR检测circRNA、miRNA和mRNA的表达;采用蛋白质印迹、RNA下拉试验、RNA免疫沉淀和双荧光素酶评估进行机制研究。我们发现，与邻近的正常组织相比，circRNA_103948在CRC组织中的表达上调，并且与不良预后相关。敲低circRNA_103948在体外和体内均抑制CRC。从机制上讲，circRNA_103948可以直接与miR-1236 - 3 p结合，并缓解对靶TPT 1的抑制。此外，circRNA_103948抑制CRC细胞的自噬。总之，circRNA_103948敲低通过靶向miR-1236 - 3 p/TPT 1轴介导的自噬抑制CRC细胞生长。因此，circRNA_103948/miR-1236 - 3 p/TPT 1轴通过调节自噬影响CRC进展。
Circular RNA (circRNA) is implicated in many types of cancer; however, the expression and role of circRNAs in colorectal cancer (CRC) remains poorly understood. In this study, a circRNA microarray assay was performed to detect abnormally expressed circRNAs in CRC, and tissue arrays were used to determine the prognosis for CRC patients. Cell counting kit‐8, clone formation, wound healing, and transwell assays were used to evaluate cell functions in vitro, and a mouse subcutaneous tumor model was designed for in vivo analysis. Autophagy was observed using confocal laser scanning and transmission electron microscopy. The expression of circRNA, miRNA, and mRNA was detected using qPCR; western blot, RNA pull‐down assay, RNA immunoprecipitation, and dual luciferase assessment were applied for mechanistic studies. We found that circRNA_103948 expression is upregulated in CRC tissues, compared with adjacent normal tissues, and associated with poor prognosis. Knockdown of circRNA_103948 suppressed CRC both in vitro and in vivo. Mechanistically, circRNA_103948 could directly bind to miR‐1236‐3p and relieve suppression of the target TPT1. Furthermore, circRNA_103948 inhibited autophagy of CRC cells. Taken together, circRNA_103948 knockdown inhibited CRC cell growth by targeting miR‐1236‐3p/TPT1 axis–mediated autophagy. Thus, the circRNA_103948/miR‐1236‐3p/TPT1 axis affects CRC progression via modulation of autophagy.