Foxj1 regulates asymmetric gene expression during left-right axis patterning in mice.
Foxj1 regulates asymmetric gene expression during left-right axis patterning in mice.
复制标题
Foxj1 在小鼠左右轴模式期间调节不对称基因表达。
DOI：
10.1016/j.bbrc.2004.09.207
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Hackett,BrianP
中科院分区：
文献类型：
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作者：
Zhang,Min;Bolfing,MaryF;Knowles,HeatherJ;Karnes,Hope;Hackett,BrianP
具有foxj 1基因靶向突变的小鼠表现出胚胎心管的D环或L环。Foxj 1在胚胎结的腹侧细胞中表达，先于其他基因的左右不对称表达。尽管在foxj 1 −/−小鼠中没有9+2纤毛，但在foxj 1 −/−胚胎的节点中存在9+0纤毛。在foxj 1 −/−胚胎中，TGF-β家族成员nodal和同源异型盒家族成员pitx 2的表达模式是随机的。在任何foxj 1 −/−早期体节期胚胎中均未观察到TGF-β家族成员lefty-2的表达。因此，Foxj 1在左右轴模式形成的早期起作用，并调节不对称的基因表达。这种调节似乎不是Foxj 1和所研究基因之间直接相互作用的结果。
Mice with a targeted mutation of the foxj1 gene demonstrate either D- or L-looping of the embryonic cardiac tube. Foxj1 is expressed in ventral cells of the embryonic node prior to asymmetric, left–right expression of other genes. Despite an absence of 9+2 cilia in foxj1−/−mice, 9+0 cilia are present in the node of foxj1−/−embryos. In foxj1−/−embryos, the patterns of expression of the TGF-β family member nodal and the homeobox family member pitx2 are randomized. No expression of the TGF-β family member lefty-2 is observed in any foxj1−/−early somite stage embryos. Foxj1 thus acts early in left–right axis patterning and regulates asymmetric gene expression. This regulation does not appear to be the result of a direct interaction between Foxj1 and the genes examined.