Cytochalasin B induces cellular DNA fragmentation
Cytochalasin B induces cellular DNA fragmentation
复制标题
细胞松弛素 B 诱导细胞 DNA 断裂
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
Belkis Landa‐Gonzalez
中科院分区：
文献类型：
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作者：
M. Kolber;Kay O. Broschat;Belkis Landa‐Gonzalez
在许多生物情况下，可以诱导细胞DNA片段化，而不会造成质膜损伤。真菌的代谢物，细胞松弛素B，能够改变与DNA合成有关的许多细胞功能。在这项工作中，证明了细胞松弛素B能够在许多细胞系中诱导DNA片段化。这种DNA断裂发生在质膜裂解之前和一段时间内。作用于微丝的细胞松弛素E和绒毛蛋白也会导致DNA断裂。佛波二丁酸酯是一种二酰基甘油类似物，能够以剂量依赖的方式抑制细胞松弛素B诱导的DNA断裂。这些发现支持细胞松弛素B通过其对肌动蛋白细丝的作用而诱导DNA片段化的解释。FASEB J.4：3021-3027；1990。
Cellular DNA fragmentation can be induced in many biological instances without plasma membrane damage. The fungal metabolite, cytochalasin B, is capable of modifying numerous cellular functions related to DNA synthesis. In this work it is demonstrated that cytochalasin B is capable of inducing DNA fragmentation in a number of cells lines. This DNA fragmentation occurs before plasma membrane lysis and over a period of hours. Cytochalasin E and villin, agents that act on the microfilaments, also induce DNA fragmentation. Phorbol dibutyrate, a diacylglyceral analog, is able to inhibit cytochalasin B‐induced DNA fragmentation in a dose‐dependent fashion. These findings support the interpretation that cytochalasin B is inducing DNA fragmentation via its effect on the actin filaments.— Kolber, M. A., Broschat, K. O., Landa‐Gonzalez, B. Cytochalasin B induces cellular DNA fragmentation. FASEB J. 4: 3021‐3027; 1990.