MOLECULAR-CLONING AND EXPRESSION OF NEUROLEUKIN, A NEUROTROPHIC FACTOR FOR SPINAL AND SENSORY NEURONS
MOLECULAR-CLONING AND EXPRESSION OF NEUROLEUKIN, A NEUROTROPHIC FACTOR FOR SPINAL AND SENSORY NEURONS
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DOI：
10.1126/science.3764429
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发表时间：
1986-10-31
期刊：
影响因子：
56.9
通讯作者：
YIN, HS
中科院分区：
文献类型：
--
作者：
GURNEY, ME;HEINRICH, SP;YIN, HS
从小鼠唾液腺中分离、克隆了一种新的56,000道尔顿生长因子，并在猴COS细胞中表达。这种蛋白质是一种神经营养因子，也是凝集素刺激的T细胞的淋巴因子产物。因此，该因子被命名为神经白细胞。脑白质促进胚胎脊髓神经元亚群在培养中的存活，其中可能包括骨骼运动神经元。神经白细胞也支持培养的感觉神经元存活，这些感觉神经元对神经生长因子不敏感，但对交感或副交感神经元没有影响。神经白细胞的氨基酸序列与引起获得性免疫缺陷综合征的逆转录病毒HTLV-III/LAV外膜蛋白的一个高度保守的区域部分同源。
A novel 56,000-dalton growth factor found in mouse salivary gland was purified, molecularly cloned, and expressed in monkey COS cells. The protein is a neurotrophic factor and also, surprisingly, a lymphokine product of lectin-stimulated T cells. The factor was therefore named neuroleukin. Neuroleukin promotes the survival in culture of a subpopulation of embryonic spinal neurons that probably includes skeletal motor neurons. Neuroleukin also supports the survival of cultured sensory neurons that are insensitive to nerve growth factor, but has no effect on sympathetic or parasympathetic neurons. The amino acid sequence of neuroleukin is partly homologous to a highly conserved region of the external envelope protein of HTLV-III/LAV, the retrovirus that causes acquired immune deficiency syndrome.