Human and mouse cementum proteins immunologically related to enamel proteins.
Human and mouse cementum proteins immunologically related to enamel proteins.
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人和小鼠的牙骨质蛋白在免疫学上与牙釉质蛋白相关。
DOI：
10.1016/0304-4165(89)90021-4
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Zeichner-David,M
中科院分区：
文献类型：
--
作者：
Slavkin,HC;Bessem,C;Fincham,AG;BringasJr,P;Santos,V;Snead,ML;Zeichner-David,M
对人和小鼠牙骨质中与釉蛋白和釉原蛋白免疫相关的脱细胞蛋白进行了SDS-聚丙烯酰胺凝胶电泳、免疫印迹和氨基酸组成分析。在该分析中，使用抗小鼠釉原蛋白、抗人釉蛋白和合成肽（例如，-LPPHPGHPGYIC-）抗体显示与分子量为72 000 Da（72 kDa）的牙冠衍生的釉蛋白、釉质蛋白（26 kDa）以及四种人牙骨质蛋白（72、58、50和26 kDa）和两种小鼠牙骨质蛋白（72和26 kDa）交叉反应。每种抗体识别牙根衍生的牙骨质多肽，其与牙冠衍生的釉质蛋白共享一个或多个表位。冠源性和根源性釉质相关蛋白的分子量和等电点相似。人和小鼠牙骨质蛋白的分析揭示了富含谷氨酰、丝氨酸、甘氨酸、丙氨酸、脯氨酸、缬氨酸和亮氨酸残基的特征性氨基酸组成;与主要釉质蛋白釉原蛋白相比，牙骨质蛋白中脯氨酸、组氨酸和甲硫氨酸含量低。人类和小鼠推定的中间牙骨质蛋白似乎代表了一个独特的类釉质相关蛋白。此外，这些结果支持的假设，上皮根鞘上皮细胞表达几个牙骨质蛋白免疫相关的典型釉质蛋白。
SDS-phoyacrylamide gel electrophoresis, immunoblot and amino acid composition analyses were applied to human and mouse acellular cementum proteins immunologically related to enamelins and amelogenins. In this analysis, anti-mouse amelogenin, anti-human enamelin and synthetic peptide (e.g., -LPPHPGHPGYIC-) antibodies were shown to cross-react with tooth crown-derived enamelin with a molecular mass of 72 000 Da (72 kDa), amelogenius (26 kDa), and also to four human cementum proteins (72, 58, 50 and 26 kDa) and two mouse cementum proteins (72 and 26 kDa). Each of the antibodies recognized tooth root-derived cementum polypeptides which share one or more epitopes with tooth crown-derived enamel proteins. The molecular mass and isoelectric points for crown-derived and root-derived enamel-related proteins were similar. Analysis of human and mouse cementum proteins revealed a characteristic amino acid composition enriched in glutamyl, serine, glycine, alanine, proline, valine and leucine residues; compared to the major enamel protein amelogenin, cementum proteins were low in proline, histidine and methionine. The human and mouse putative intermediate cementum proteins appear to represent a distinct class of enamel-related proteins. Moreover, these results support the hypothesis that epithelial root sheath epithelia express several cementum proteins immunologically related to canonical enamel proteins.